Table 1 Primers used for genus and RD9 typing of isolated mycobacteria
Primer name | Primer sequence(5′ to 3′) | Product size |
|---|---|---|
MYCGEN-F | AGAGTTTGATCCTGGCTCAG | 1030 bp |
MYCGEN-R | TGCACACAGGCCACAAGGGA | |
MYCAV-R | ACCAGAAGACATGCGTCTTG | 180 bp |
MYCINT-F | CCTTTAGGCGCATGATGTCTTTA | 850 bp |
TB1-F | GAACAATCCGGAGTTGACAA | 372 bp |
TB-R | AGCACGCTGTCAATCATGTA | |
RD9_IntR | CTGGACCTCGATGACCACTC | 396 bp (presence of RD9) |
RD9_FlankF | AACACGGTCACGTTGTCGTG | 575 bp (absence of RD9) |
RD9_FlankR | CAAACCAGCAGCTGTCGTTG |