Table 1 Effect of sperm treated with colloids at different oocyte:sperm ratios on in vitro embryo development (mean ± SEM)
From: Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro
Treatment Ratio oocyte: spermatozoa | n | Spermatozoa bound to the ZP | Polyspermic oocytes (%) | Fertilization rate (%) | Cleavage rate (48 hpi)% | Blastocyst rate (day 7)% |
|---|---|---|---|---|---|---|
No. spermatozoa (over non-cleaved oocytes) | (over non-cleaved oocytes) | Over No. Presumptive zygotes | No. embryos/No. presumptive zygotes | No. blastocysts/No. presumptive zygotes | ||
Control 1:50 | 6 | 9.4 ± 6.5 a | 8.2 ± 3.0 a | 59.3 ± 4.4 a | 55.7 ± 4.0 a | 12.5 ± 2.0 a |
(344/128) | (11/126) | (175/292) | (164/292) | (37/292) | ||
Control 1:100 | 8 | 5.1 ± 0.6 a | 27.6 ± 6.2 b | 67.0 ± 5.1 ab | 55.1 ± 4.9 a | 17.7 ± 3.7 a |
(758/145) | (37/141) | (215/317) | (174/317) | (55/317) | ||
SLC 1:50 | 6 | 2.9 ± 0.6 a | 15.3 ± 5.4 a | 62.9 ± 5.5 a | 56.0 ± 4.2 a | 16.0 ± 4.1 a |
(328/136) | (16/134) | (183/304) | (167/304) | (47/304) | ||
SLC 1:100 | 8 | 6.7 ± 1.2 a | 36.4 ± 7.9 b | 73.5 ± 3.4 ab | 56.2 ± 3.2 a | 15.8 ± 2.2 a |
(944/135) | (51/132) | (244/315) | (176/315) | (49/315) | ||
SLC 1:300 | 7 | 20.5 ± 2.1 b | 66.5 ± 6.7 c | 78.4 ±2.8 b | 31.0 ± 4.3 b | 5.2 ± 1.4 b |
(4118/194) | (133/194) | (224/291) | (91/291) | (15/291) |