Genes involved in carnitine synthesis and carnitine uptake are up-regulated in the liver of sows during lactation

Rosenbaum, Susann; Ringseis, Robert; Most, Erika; Hillen, Sonja; Becker, Sabrina; Erhardt, Georg; Reiner, Gerald; Eder, Klaus

doi:10.1186/1751-0147-55-24

Acta Veterinaria Scandinavica

Table 1 Characteristics of primers and primer performance data used for qPCR

From: Genes involved in carnitine synthesis and carnitine uptake are up-regulated in the liver of sows during lactation

Gene symbol	Forward primer (from 5^′to 3^′)	Product size (bp)	NCBI GenBank	Slope	R2^#	Efficiency*	M
Gene symbol	Reverse primer (from 5^′to 3^′)	Product size (bp)	NCBI GenBank	Slope	R2^#	Efficiency*	M
Reference genes
RSP9	GTCGCAAGACTTATGTGACC	325	XM_003356050	−0.28	0.999	1.91	0.053
RSP9	AGCTTAAAGACCTGGGTCT	325	XM_003356050	−0.28	0.999	1.91	0.053
ATP5G1	CAGTCACCTTGAGCCGGGCGA	94	NM_001025218	−0.30	0.998	1.99	0.054
ATP5G1	TAGCGCCCCGGTGGTTTGC	94	NM_001025218	−0.30	0.998	1.99	0.054
GSR	AGCGCGATGCCTACGTGAGC	175	AY368271	−0.29	0.997	1.94	0.055
GSR	GGTACGCCGCCTGTGGCAAT	175	AY368271	−0.29	0.997	1.94	0.055
ACTB	GACATCCGCAAGGACCTCTA	205	XM_003124280	−0.32	0.992	2.10	0.064
ACTB	ACATCTGCTGGAAGGTGGAC	205	XM_003124280	−0.32	0.992	2.10	0.064
SHAS2	GAAAAGGCTAACCTACCCTG	218	NM_214053	−0.21	0.996	1.65	0.076
SHAS2	TGTTGGACAAGACCAGTTGG	218	NM_214053	−0.21	0.996	1.65	0.076
Target genes
ACOX1	CTCGCAGACCCAGATGAAAT	218	AF185048	−0.28	0.995	1.90
ACOX1	TCCAAGCCTCGAAGATGAGT	218	AF185048	−0.28	0.995	1.90
ALDH9A1	GCTGCTGGCCGAAATCTATA	215	XM_001924860	−0.29	0.999	1.94
ALDH9A1	CACACTTCCAGTGAAGGAGA	215	XM_001924860	−0.29	0.999	1.94
BBOX1	GTGCCGAAAGCTCAAGGAAAAA	342	XM_003122909	−0.31	0.985	2.06
BBOX1	CTCTGCCGGCCGTGAAGTAAC	342	XM_003122909	−0.31	0.985	2.06
CYP4A24	GGTTTGCTCCTGTTGAATGG	121	NM_214424	−0.29	0.999	1.96
CYP4A24	GCATCACTTGGACAGACTTG	121	NM_214424	−0.29	0.999	1.96
FABP4	CACCAGGAAGGTGGCTGGCA	197	NM_001002817	−0.30	0.999	1.98
FABP4	CCTGTACCAGGGCGCCTCCA	197	NM_001002817	−0.30	0.999	1.98
FGF21	GAAGCCAGGGGTCATTCAAA	149	NM_001163410	−0.31	0.999	2.03
FGF21	GGTAAACGTTGTAGCCATCC	149	NM_001163410	−0.31	0.999	2.03
HMGCS2	GGACCAAACAGACCTGGAGA	198	NM_214380	−0.32	0.994	2.07
HMGCS2	ATGGTCTCAGTGCCCACTTC	198	NM_214380	−0.32	0.994	2.07
SLC22A5	TGACCATATCAGTGGGCTA	384	XM_003123912	−0.30	0.995	2.00
SLC22A5	AGTAGGGAGACAGGATGCT	384	XM_003123912	−0.30	0.995	2.00
SLC25A20	GCAAAGCCCATTAGCCCTCT	312	XM_003483178	−0.28	0.998	1.89
SLC25A20	GAGCACATCCTCTGGGTGTT	312	XM_003483178	−0.28	0.998	1.89
TMLHE	GCACCATACAGCCTCCAAGT	221	XM_003135511	−0.35	0.995	1.79
TMLHE	TGGTCTCATCCAGACGAACA	221	XM_003135511	−0.35	0.995	1.79

^#Coefficient of determination of the standard curve. *The efficiency is determined by [10^-slope].

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ISSN: 1751-0147

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