Quantification of Mycobacterium avium subspecies in pig tissues by real-time quantitative PCR

Table 1 Quantification of Mycobacterium cells in lymph nodes originating from slaughter pigs suffering from a mycobacterial infection

Sample	Description	qPCR cell equivalents* of mycobacterial DNA g-1 tissue
Pig 4, sample1.	Lesion	3 × 10⁶
Pig 4, sample 2.	outside the lesion part	below detection limit
Pig 9–5577, sample1.	Lesion	2 × 10⁶
Pig 9–5577, sample 2.	outside the lesion part	below detection limit
Pig Austria 3, sample 1.	Lesion	2 × 10⁷
Pig Austria 3, sample 2.	outside the lesion part	2 × 10⁴
Pig 187, sample 1.	Lesion	below detection limit
Pig 187, sample 2.	outside the lesion part	below detection limit

*Cell equivalent calculated based on the copy assumed number of one 16S rRNA gene per mycobacterial cell.

ISSN: 1751-0147