Mutant prevention concentration of orbifloxacin: comparison between Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin

Shimizu, Takae; Harada, Kazuki; Kataoka, Yasushi

doi:10.1186/1751-0147-55-37

Acta Veterinaria Scandinavica

Table 1 MICs and MPCs of orbifloxacin and QRDR mutations in the gyrA , parC , and grlA genes of the original stains and mutants used in the study

From: Mutant prevention concentration of orbifloxacin: comparison between Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin

Parent strains and mutants^a	MIC (μg/ml)	MIC (+EPI)^b(μg/ml)	QRDR mutation^c		MPC (μg/ml)	MPC /MIC	C_max/MPC^d	AUC /MPC^d
Parent strains and mutants^a	MIC (μg/ml)	MIC (+EPI)^b(μg/ml)	*gyrA*	*parC( grlA*)	MPC (μg/ml)	MPC /MIC	C_max/MPC^d	AUC /MPC^d
E. coli
E1	0.063	<0.015	wt	wt	1	16	6.9	42.9
E2	0.063	<0.015	wt	wt	0.5	8	13.8	85.8
E3	0.063	<0.015	wt	wt	1	16	6.9	42.9
E4	0.125	<0.015	wt	wt	2	16	3.45	21.45
E5	0.125	<0.015	wt	wt	2	16	3.45	21.45
E6	0.25	<0.015	wt	wt	1	4	6.9	42.9
E7	0.5	0.063	S83L	wt	16	32	0.43	2.68
E8	1	0.063	D87N	wt	8	8	0.86	5.36
E9	2	0.125	D87N	wt	8	4	0.86	5.36
E10	2	0.125	S83L	wt	32	16	0.22	1.34
EM1 (0.5)	1	0.125	S83L	wt	-	-
EM2 (0.25)	1	0.125	S83L	wt	-	-
EM3 (0.5)	1	0.125	S83L	wt	-	-
EM4 (1)	2	0.125	S83L	wt	-	-
EM5 (1)	2	0.25	S83L	wt	-	-
EM6 (0.5)	2	0.125	S83L	wt	-	-
EM7 (8)	4	0.125	S83L	wt	-	-
EM8 (4)	4	0.25	D87N	wt	-	-
EM9 (4)	8	0.25	D87N	wt	-	-
EM10 (16)	4	0.125	S83L	wt	-	-
P. aeruginosa
P1	1	0.015	wt	wt	32	32	0.22	1.34
P2	1	0.031	wt	wt	16	16	0.43	2.68
P3	1	0.063	wt	wt	32	32	0.22	1.34
P4	2	0.125	wt	wt	64	32	0.11	0.67
P5	2	0.063	wt	wt	32	16	0.22	1.34
P6	2	0.125	wt	wt	32	16	0.22	1.34
P7	2	0.031	wt	wt	64	16	0.11	0.67
P8	2	0.063	wt	wt	128	64	0.05	0.34
P9	4	0.25	wt	wt	64	16	0.11	0.67
P10	4	0.125	wt	wt	64	16	0.11	0.67
PM1 (16)	32	0.063	wt	wt	-	-
PM2 (8)	16	0.25	wt	wt	-	-
PM3 (16)	16	0.5	wt	wt	-	-
PM4 (32)	32	1	T83I	wt	-	-
PM5 (16)	32	0.25	wt	wt	-	-
PM6 (16)	64	0.125	wt	wt	-	-
PM7 (32)	64	1	T83I	wt	-	-
PM8 (64)	64	0.125	T83I	wt	-	-
PM9 (32)	128	2	T83I	wt	-	-
PM10 (32)	16	0.25	wt	wt	-	-
S. pseudintermedius
S1	0.25	0.25	wt	wt	2	8	3.45	21.45
S2	0.25	0.5	wt	wt	2	4	3.45	21.45
S3	0.25	0.5	wt	wt	8	16	0.86	5.36
S4	0.5	0.5	wt	wt	2	8	3.45	21.45
S5	0.5	0.5	wt	wt	4	8	1.73	10.73
S6	0.5	0.5	wt	wt	4	4	1.73	10.73
S7	1	1	wt	S80I	128	128	0.05	0.34
S8	1	1	wt	S80I	128	128	0.05	0.34
S9	1	1	wt	S80I	64	64	0.11	0.67
S10	1	1	wt	S80I	16	16	0.43	2.68
SM1 (1)	1	1	wt	wt	-	-
SM2 (1)	0.5	0.5	wt	wt	-	-
SM3 (4)	1	1	wt	wt	-	-
SM4 (1)	2	1	wt	wt	-	-
SM5 (2)	0.5	0.5	wt	wt	-	-
SM6 (2)	1	2	wt	S80I	-	-
SM7 (64)	64	128	S84W	S80I	-	-
SM8 (64)	64	128	S84L	S80I	-	-
SM9 (32)	8	8	wt	S80I	-	-
SM10 (8)	32	32	S84L	S80I	-	-

^a Number in parenthesis indicates the orbifloxacin concentration (μg/ml) supplemented in the agar from which mutants were derived.
^b Minimum inhibitory concentrations (MICs) in the presence of efflux pump inhibitors (EPIs).
^cparC of E. coli and P. aeruginosa, and grlA of S. pseudintermedius; wt, wild type; S83L, Ser-83 → Leu; D87N, Asp-87 → Asn; T83I, Thr-83 → Ile; S80I, Ser-80 → Ile; S84W, Ser84 → Trp; S84L, Ser84 → Leu.
^d Data for maximum concentration (C_max: 6.9 mg/l) and area under the concentration time-curve (AUC: 42.9 mg.h/l) of orbifloxacin (dose of 7.5 mg/kg) are from reference [16].

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ISSN: 1751-0147

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