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Table 1 MICs and MPCs of orbifloxacin and QRDR mutations in the gyrA , parC , and grlA genes of the original stains and mutants used in the study

From: Mutant prevention concentration of orbifloxacin: comparison between Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin

Parent strains and mutantsa MIC (μg/ml) MIC (+EPI)b(μg/ml) QRDR mutationc MPC (μg/ml) MPC /MIC Cmax/MPCd AUC /MPCd
gyrA parC( grlA)
E. coli
E1 0.063 <0.015 wt wt 1 16 6.9 42.9
E2 0.063 <0.015 wt wt 0.5 8 13.8 85.8
E3 0.063 <0.015 wt wt 1 16 6.9 42.9
E4 0.125 <0.015 wt wt 2 16 3.45 21.45
E5 0.125 <0.015 wt wt 2 16 3.45 21.45
E6 0.25 <0.015 wt wt 1 4 6.9 42.9
E7 0.5 0.063 S83L wt 16 32 0.43 2.68
E8 1 0.063 D87N wt 8 8 0.86 5.36
E9 2 0.125 D87N wt 8 4 0.86 5.36
E10 2 0.125 S83L wt 32 16 0.22 1.34
EM1 (0.5) 1 0.125 S83L wt - -   
EM2 (0.25) 1 0.125 S83L wt - -   
EM3 (0.5) 1 0.125 S83L wt - -   
EM4 (1) 2 0.125 S83L wt - -   
EM5 (1) 2 0.25 S83L wt - -   
EM6 (0.5) 2 0.125 S83L wt - -   
EM7 (8) 4 0.125 S83L wt - -   
EM8 (4) 4 0.25 D87N wt - -   
EM9 (4) 8 0.25 D87N wt - -   
EM10 (16) 4 0.125 S83L wt - -   
P. aeruginosa
P1 1 0.015 wt wt 32 32 0.22 1.34
P2 1 0.031 wt wt 16 16 0.43 2.68
P3 1 0.063 wt wt 32 32 0.22 1.34
P4 2 0.125 wt wt 64 32 0.11 0.67
P5 2 0.063 wt wt 32 16 0.22 1.34
P6 2 0.125 wt wt 32 16 0.22 1.34
P7 2 0.031 wt wt 64 16 0.11 0.67
P8 2 0.063 wt wt 128 64 0.05 0.34
P9 4 0.25 wt wt 64 16 0.11 0.67
P10 4 0.125 wt wt 64 16 0.11 0.67
PM1 (16) 32 0.063 wt wt - -   
PM2 (8) 16 0.25 wt wt - -   
PM3 (16) 16 0.5 wt wt - -   
PM4 (32) 32 1 T83I wt - -   
PM5 (16) 32 0.25 wt wt - -   
PM6 (16) 64 0.125 wt wt - -   
PM7 (32) 64 1 T83I wt - -   
PM8 (64) 64 0.125 T83I wt - -   
PM9 (32) 128 2 T83I wt - -   
PM10 (32) 16 0.25 wt wt - -   
S. pseudintermedius
S1 0.25 0.25 wt wt 2 8 3.45 21.45
S2 0.25 0.5 wt wt 2 4 3.45 21.45
S3 0.25 0.5 wt wt 8 16 0.86 5.36
S4 0.5 0.5 wt wt 2 8 3.45 21.45
S5 0.5 0.5 wt wt 4 8 1.73 10.73
S6 0.5 0.5 wt wt 4 4 1.73 10.73
S7 1 1 wt S80I 128 128 0.05 0.34
S8 1 1 wt S80I 128 128 0.05 0.34
S9 1 1 wt S80I 64 64 0.11 0.67
S10 1 1 wt S80I 16 16 0.43 2.68
SM1 (1) 1 1 wt wt - -   
SM2 (1) 0.5 0.5 wt wt - -   
SM3 (4) 1 1 wt wt - -   
SM4 (1) 2 1 wt wt - -   
SM5 (2) 0.5 0.5 wt wt - -   
SM6 (2) 1 2 wt S80I - -   
SM7 (64) 64 128 S84W S80I - -   
SM8 (64) 64 128 S84L S80I - -   
SM9 (32) 8 8 wt S80I - -   
SM10 (8) 32 32 S84L S80I - -   
  1. a Number in parenthesis indicates the orbifloxacin concentration (μg/ml) supplemented in the agar from which mutants were derived.
  2. b Minimum inhibitory concentrations (MICs) in the presence of efflux pump inhibitors (EPIs).
  3. cparC of E. coli and P. aeruginosa, and grlA of S. pseudintermedius; wt, wild type; S83L, Ser-83 → Leu; D87N, Asp-87 → Asn; T83I, Thr-83 → Ile; S80I, Ser-80 → Ile; S84W, Ser84 → Trp; S84L, Ser84 → Leu.
  4. d Data for maximum concentration (Cmax: 6.9 mg/l) and area under the concentration time-curve (AUC: 42.9 mg.h/l) of orbifloxacin (dose of 7.5 mg/kg) are from reference [16].