Figure 2

Inhibition of the binding between holoferritin and purified fibrinogen or fibrinogen in heat-treated plasma by hemin, metal free-, Sn-, or Zn-PPIX. Aliquots (100 μL) of 10 μg/mL of purified horse fibrinogen (A) or heated-horse plasma sample (B) as described in “Figure 1” were added to wells of holo-ferritin-coated plate (1 pmol/well) and hemin (solid bar), Sn- (gray bar), Zn- (dotted bar), or metal free-PPIX (open bar) was added to a final concentration of 10 μmol/L. Fibrinogen bound to the wells was detected as already described in “Figure 1”. Binding activity (%) was determined by comparison to the control (100%) in the absence of each inhibitor. a: P < 0.01 compared to the binding activity in the presence of hemin; b: P < 0.01 compared to the binding activity in the presence of Sn-PPIX; c: P < 0.01 compared to the binding activity in the presence of Zn-PPIX. Each data represents mean ± SD of four determinations.