Figure 1

Detection of vaccine plasmids (A) and expression of the vaccine-encoding genes (B and C) in fish tissues. (A) Flounder were vaccinated with pSagE (lanes 3, 5, 7, and 9) or pCN3 (lanes 2, 4, 6, and 8). At 7 days post-vaccination, DNA and RNA were extracted from muscle (lanes 2 and 3), spleen (lanes 4 and 5), liver (lanes 6 and 7), and kidney (lanes 8 and 9) and used for PCR (A) and RT-PCR (B and C) analysis. PCR was performed using primers specific to pSagE or pCN3. RT-PCR was performed using primers specific to sagE (B) or α-tubulin (internal reference) (C). Lane 1, DNA markers.