Figure 3From: SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular regionExpression of immune genes in vaccinated fish. Flounder were vaccinated with pSagE, pSagEECR, or pCN3 (control). Spleen was taken from the fish at 24 h post-challenge. Total RNA was extracted from the spleen and used for quantitative real time RT-PCR. For each gene, the mRNA level of the control fish was set as 1. Data are presented as means ± SE (N = 3). *P < 0.05; **P < 0.01.Back to article page