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Table 1 Primers used in this study

From: SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region

Primer

Sequences (5′→ 3′)a

CNF1

CTTGCGTTTCTGATAGGCACCTA

CNR1

TGCGGGCCTCTTCGCTATT

ECRF1

CCCGGG ACCGCCATGCGTTGCTTTCAAAA (SmaI)

ECRR1

GCTCCCGGG TAAGATAGCAAACCAT (SmaI)

ECRF2

CCCGGG ATGCGTTGCTTTCAAAA (SmaI)

SagEF1

CCCGGG ACCACCATGATTTTTGGAAAAAGTAGTAATGGA (SmaI)

SagER1

GCCCGGG CCTTCTTACCTTTGACTGAT (SmaI)

SagEF2

ATGGAAAACTTCTCACAGGACTC

SagECR

GCTAACGCATTCAACCACAAA

  1. aUnderlined nucleotides are restriction sites of the enzymes indicated in the brackets at the ends.
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Acta Veterinaria Scandinavica

ISSN: 1751-0147