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Table 3 Results of testing of the heterologous sera in the H5 inhibition ELISAs

From: An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies

Immunisation

PMV8 (n = 8)

H16N3 (n = 10)

H1N2 (n = 7)

H9N9 (n = 7)

H10N4 (n = 16)

H7N1 (n = 20)

H7N7 (n = 12)

H5N7ag-ELISA

6.3 (13.5)

21.5 (31.0)

19.7 (26.7)

19.1 (27.9)

13.3 (18.3)

17.6 (26.6)

42.3 (55.8)

 

16.2 (28.0)

 

H5N2ag-ELISA

8.4 (11.9)

11.3 (15.1)

12.9 (18.7)

7.4 (11.7)

2.8 (12.2)

3.6 (18.6)

3.0 (17.8)

 

6.0 (18.7)

 

2 subsequent ELISA

5.6 (12.5)

11.3 (15.1)

12.9 (18.7)

7.4 (11.7)

2.7 (11.3)

3.6 (18.6)

3.0 (17.8)

 

5.7 (18.3)

 
  1. Values are given as mean inhibition percentages and mean + 2 standard deviations (in brackets). Two subsequent ELISA implies that the first ELISA is done using the H5N7 antigen and then a secondary ELISA is performed with the H5N2 antigen. The minimum value for the two assays is used as the final result.
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Acta Veterinaria Scandinavica

ISSN: 1751-0147