Table 1 Characteristics of primers used for qPCR
From: Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep
Gene | Forward primer (3′-5′) | Reverse primer (5′-3′) | Product length (bp) | Tm(°C) | NCBI Genbank |
|---|---|---|---|---|---|
Reference genes | |||||
B2M | GCGTATTCCAGAGGTCCAGG | CGGCAGCTGTACTGATCCTT | 234 | 60 | NM_001009284 |
GAPDH | GGCGTGAACCACGAGAAGTA | GCAGGGATGATGTTTTGGGC | 227 | 60 | AF022183 |
MDH1 | TACGTGTTCCCTGGAGTTGC | TGCTTCCTTGTTTGGGGGTT | 249 | 57 | NM_001135220 |
RPL19 | AGCCTGTGACTGTCCATTCC | TTCTCGGGCATTCGAGCATT | 118 | 57 | JN811679 |
RPS26 | ACAACGGTCGTGCCAAAAAG | AAATCGGGGTGGAGGTGTTC | 284 | 57 | NM_001009435 |
YWHAZ | AGACGGAAGGTGCTGAGAAA | TGGGGATCAAGAACTTTTCCAA | 120 | 57 | JN811681 |
Target genes | |||||
COX5A | GCTCGCTGGGTGACATACTT | ACCTCTAGGATGCGAACTGC | 173 | 60 | AF233074 |
COX6A1 | TGCAGCTGAGTCGGTGTATG | GAACTCGGGTCTCTCCTCCT | 161 | 60 | GU585577 |
CPT1B | GACGTTTCCATGGGACTGGT | GCCAGCGTCTCCATTCGATA | 389 | 60 | NM_001009259 |
MHCI | TCGTCAAGGCCACAATTTTG | CTGTCGCAACACCTGGTCCT | 100 | 60 | AB058898 |
MHCIIA | AAGCCTTTTGATGCCAAGACAT | TTCACCGTCACTTTCCCACC | 100 | 60 | AB058896 |
MHCIIX | CTTCGTGGCGGACCCTAAG | CAGTTACTGTCGCCCCAGCT | 100 | 60 | AB058897 |
PPARD | TCAGCGTGCACGTCTTCTAC | CAGGAATTCCCGGGTGACAA | 230 | 59 | XM_004018769 |
PPARGC1A | GGTGACCATGACTATTGTCAG | CTCGGATTTCCTGGTCTTGAA | 216 | 58 | XM_004009738 |
PPARGC1B | CTGGACCGAGTTCTCCATCC | CACGTGCCCTTTCACCTGCA | 244 | 61 | XM_004008965 |
SDHA | GTTTGAGCAGCACTGGAGGA | AGTCGGTCTCGTTCAAAGTCC | 110 | 60 | DQ386895 |
SLC25A20 | CCGAGGGATCTACAAGGGGA | CCTTCATCCCGGATCAGCTC | 288 | 61 | NM_001127277 |
VEGFA | GGACATCTTCCAGGAGTACC | GCATGGTGATGTTGAACTCCT | 137 | 58 | EU857623 |