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Table 2 qPCR performance data

From: Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep

Gene

Slope

R2#

Efficiency*

B2M

−3.20

0.999

2.05

COX5A

−3.22

0.999

2.04

COX6A1

−2.99

0.997

2.16

CPT1B

−3.79

0.996

1.84

GAPDH

−2.97

0.999

2.17

MDH1

−3.21

0.999

2.05

MHCI

−3.37

1.000

1.98

MHCIIA

−3.21

0.998

2.05

MHCIIX

−3.29

0.999

2.01

PPARD

−3.05

0.967

2.13

PPARGC1A

−3.34

0.999

1.99

PPARGC1B

−3.29

0.956

2.01

RPL19

−3.31

0.997

2.00

RPS26

−3.72

0.998

1.86

SDHA

−3.12

0.999

2.09

SLC25A20

−3.81

0.980

1.83

VEGFA

−3.31

0.997

2.00

YWHAZ

−3.34

0.993

1.99

  1. #Coefficient of determination of the standard curve.
  2. *The efficiency is determined by [10(−1/-slope].