Table 1 Cytokine primers for RT-PCR
From: Evaluation of adverse reactions in dogs following intravenous mesenchymal stem cell transplantation
Primer set | Primer sequence | 5′ fluorophore | Probe sequence | 3′ quencher |
|---|---|---|---|---|
GAPDHa | Forward TCAACGGATTTGGCCGTATTGG | FAM | CAGGGCTGCTTTTAACTCTGGCAAAGTGGA | BHQ-1 |
Reverse TGAAGGGGTCATTGATGGCG | ||||
TNF-αb | Forward GAGCCGACGTGCCAATG | FAM | CGTGGAGCTGACAGACAACCAGCTG | BHQ-1 |
Reverse CAACCCATCTGACGGCACTA | ||||
IL-4a | Forward GCTCCAAAGAACACAAGCGA | FAM | TGCAGAGCTGCTACTGTACTGCGGC | BHQ-1 |
Reverse CATGCTGCTGAGGTTCCTGT | ||||
IL-6a | Forward CTCTCCACAAGCGCCTTCTC | FAM | TGGGGCTGCTCCTGGTGATG | BHQ-1 |
Reverse TGAAGTGGCATCATCCTTGG | ||||
IL-10a | Forward CGACCCAGACATCAAGAACC | FAM | TCCCTGGGAGAGAAGCTCAAGACCC | BHQ-1 |
Reverse CACAGGGAAGAAATCGGTGA |