Figure 2

Ig-438 affinity chromatography profiles from fallow deer. Fractions were issued from fetal cotyledonary (A) and maternal caruncula (B) tissues. Column (0.7 × 5 cm, 2 ml) was previously equilibrated with PBS 0.05 M containing 0.15 M NaCl (pH 7.4). Unbound proteins were eliminated after washing with PBS 0.05 M containing 0.15 M NaCl, followed by washing with PBS 0.05 M containing 0.3 M NaCl. Bound proteins were eluted by adding 0.1 M glycine solution adjusted to pH 2.8 with HCl. Before elution, 1.5 ml sodium bicarbonate buffer (0.1 M, pH 8.3) was added to each collection tube.