Figure 3
Western blot of fdPAG proteins extracted from fetal cotyledonary (FCT) and maternal caruncular (MCT) tissues. Two polyclonal antisera raised against boPAG-2 (AS#435) or caprine PAG55+62kDa (AS#706) were used as primary antiserum at 1:100 dilution. Molecular weight standards (kDa; 7 μg/lane) were loaded on the right or left position of figures. Lane 1: A.S. 40-80% fraction from FCT; Lane 2: VVA eluted peak from FCT; Lane 3: eluted peak from Sepharose 4B Ig-438 affinity chromatography from FCT; Lane 4: A.S. 40-80% from MCT; Lane 5: VVA peak from MCT; Lane 6: eluted peak from Sepharose 4B Ig-438 affinity chromatography from MCT. Fifty μg were loaded in lanes 1 and 4; 30 μg were loaded on lanes 2, 3, 6 and 7.