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Acta Veterinaria Scandinavica

Open Access

A new test for canine intestinal mucosa permeability

  • Ragnvi Hagman1Email author,
  • Pernilla Rosberg1,
  • Anas Al-Saffar2 and
  • Dominic Luc-Webb2
Acta Veterinaria Scandinavica201557(Suppl 1):O16

Published: 25 September 2015


MannitolRiboflavinLactuloseIntestinal PermeabilityPermeability Evaluation


Increases in mucosal intestinal permeability may cause pathological leakage of bacterial products, inducing inflammation. Low-grade systemic inflammation has been reported in overweight humans. Probes used for permeability evaluation in humans have not yet been evaluated in dogs.


To determine suitability of human permeability probes (riboflavin, lactulose, mannitol and sucralose) for canine obesity models.


Fourteen healthy dogs were examined as baseline group. They were given all four probes per os using human doses corrected for weight and reported to be harmless for dogs. Urine samples were collected 2, 4 and 6 h after ingesting probes. Urinary excretion was quantified.


The least squares means of mannitol proportion (excreted/ingested ± SE) was 15.6 % (± 2.0), 11.0 % (± 2.1) and 5.5 % (± 2.1) for urine sampled 2, 4 and 6 h after ingestion (p<0.0001). Hence, the majority of mannitol absorption and secretion representing upper gastrointestinal leakage was completed by 4 h. Riboflavin followed a similar temporal profile, defining 4 h as a cutoff for upper vs lower gut permeability. Probes were well tolerated.


Data obtained in the study indicates that probes used for humans can be used for intestinal permeability evaluation in dogs.

Authors’ Affiliations

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, Uppsala, Sweden


© Hagman et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.