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Acta Veterinaria Scandinavica

Open Access

Testosterone and anti-Müllerian-hormone (AMH) in lean and overweight male Labrador Retrievers

  • Elina Andersson1,
  • Josefin Söder2,
  • Katja Höglund2,
  • Ragnvi Hagman1,
  • Sara Wernersson2 and
  • Bodil Ström Holst1, 3Email author
Acta Veterinaria Scandinavica201557(Suppl 1):P1

Published: 25 September 2015


TestosteroneSerum ConcentrationStandardise SamplingHigh BodyMedian Concentration


Anti-Müllerian hormone (AMH) and testosterone are produced by the testicles. Both hormones affect sperm production, and hence fertility. In men with a high body condition score (BCS), the testosterone concentration is low.


To investigate the influence of body condition score on serum concentration of testosterone and AMH in clinically healthy male dogs. The hypothesis was that overweight dogs have lower testosterone concentrations, and lower or higher concentrations of AMH, than lean dogs.


Blood samples from 12 lean (BCS 4-5) and 16 overweight (BCS 6-8) male Labrador Retrievers were analysed. Samples were collected at the same time of the day in all dogs. AMH and testosterone concentrations were analysed with ELISAs (AMH Gen II ELISA, Beckman coulter, and testosterone ELISA, IBL International, GmbH). The Mann-Whitney U test was used for group comparisons.


Median concentration and inter-quartile range was 9.6 pg/L (7.4-14.1) for AMH and 9.7 nmol/L (6.3-15.4) for testosterone. Three testosterone results were excluded due to technical problems. There were no significant differences between lean and overweight dogs.


Serum concentrations of testosterone and AMH did not differ between lean and overweight dogs. This could be due to a lack of real difference, that the sample population was too small and the power of the study thus limited, or that the BCS was too low in the overweight dogs (only mild overweight). The concentration of testosterone, and potentially also AMH, varies throughout the day, but due to the standardised sampling, this should not affect the results.

Authors’ Affiliations

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden
Centre for Reproductive Biology in Uppsala (CRU), Swedish University of Agricultural Sciences, Uppsala, Sweden


© Andersson et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.