Skip to main content
Download PDF
  • Published:

Relationship Between ATP Content and Post Thaw Motility in Bull Semen

Sambandet mellan ATP innehåll och upptiningsmotilitet i tjursperma

Acta Veterinaria Scandinavica volume 26, pages 308–312 (1985)Cite this article

Abstract

The ATP content in frozen and thawed semen from 17 bulls was determined by a bioluminescence method.

The post thaw motility was assessed by phase contrast microscopy by subjective estimation of the percentage of sperm cells with forward motility. The concentration of spermatozoa was counted in a Bürker chamber. The correlation between ATP content and the number of sperm cells with forward motility was high.

Sammanfattning

Djupfryst sperma från 17 tjurar undersöktes efter upptining med hjälp av en bioluminescence metod på sitt ATP innehåll.

Spermiemotiliteten fastställdes i faskontrastmikroskop genom subjektiv bedömning av den procentuella andelen spermier med progressiv framåtgående rörelse. Spermiekoncentrationen framräknades med hjälp av Bürkerkammare.

Korrelationen mellan ATP innehåll och andelen spermier med progressiv framåtgående rörelse var hög.

References

  • Foulkes, J. A. & B. J. MacDonald: The relationship between ATP content and motility of bovine spermatozoa. Theriogenology 1979, 11, 313–319.

    Article  CAS  Google Scholar 

  • Mann, T.: Studies on the metabolism of semen. 1. General aspects. Occurrence and distribution of cytocrome, certain enzymes and co-enzymes. J. Biochem. 1945a, 39, 451–458.

    CAS  Google Scholar 

  • Mann, T.: Studies on the metabolism of semen. 2. Glycolysis in spermatozoa. J. Biochem. 1945b, 39, 458–165.

    CAS  Google Scholar 

  • Olsson, T., H. Gulliksson, M. Palmeborn, K. Bergström & A. Thore: Methodological aspects on the firefly luciferase assay of adenine nucleotides in whole blood and red blood cells. Scand. J. clin. Lab. Invest. 1983, 43, 657–664.

    Article  CAS  Google Scholar 

  • von Prinzen, R.: ATP-bestimmungen (Adenosin-Tri-Phosphat) mit einer Schnellmethode and frischen und tiefgefrorenen Bullenspermien (A method for the rapid determination of ATP (Adenosine-Tri-Phosphate) in fresh and frozen bull sperm). Zuchthyg. 1977, 12, 105–108,

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7039, S-750 07, Uppsala, Sweden

    L. Söderquist & K. Larsson

Authors
  1. L. Söderquist
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Larsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Söderquist, L., Larsson, K. Relationship Between ATP Content and Post Thaw Motility in Bull Semen. Acta Vet Scand 26, 308–312 (1985). https://doi.org/10.1186/BF03546531

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03546531

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147