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Adaption of Elisa for the Detection oCampylobacter Antibodies and its Application in Seroepidemiological Studies in Sheep and Cattle Herds
Tillämpning av ELISA för påvisning av antikroppar mot Campylobacter samt användning i seroepidemiologiska studier i får- och nötbesättnigar
Acta Veterinaria Scandinavica volume 26, pages 30–48 (1985)
Abstract
ELISA was adapted for the study of antigenic relations among important Campylobacters and for the presence of anti-campylo-bacter antibodies in 394 sheep and 265 cattle. Rabbit anti-C. jejuni, C. coli, G. fetus subsp. fetus and C. laridis heat-stable antigen sera were evaluated against 29 Campylobacter strains and 6 other bacteria. Anti-C. jejuni and G. coli reacted strongly with homologous antigens and weakly with C. fetus subsp. fetus, C. laridis and C. fecalis antigens. C. fetus subsp. fetus serum reacted mainly with its homologous antigen. C. laridis serum showed closer reactivity to C. jejuni than to C. fetus subsp. fetus, C. coli and C. fecalis. Insignificant cross-reactions were observed with Y. enterocolitica, S. dublin and E. aerogenes heat-stable antigens, Ewes vaccinated with C. fetus subsp. fetus bacterin showed higher ELISA titers against C. fetus subsp. fetus antigens than non-vaccinated ewes or rams. Twenty-five percent of the vaccinated animals showed titers as low as 95 % of the non-vaccinated animals. In cattle the lowest antibody titers against C. fetus subsp. fetus, C. jejuni, C. coli and C. laridis antigens were exhibited by the precolostrum sera followed by the postcolostrum and adult sera. These studies demonstrated the applicability of the ELISA test in seroepidemiological investigations concerning the distribution and significance of Campylobacter antibodies in food animal sera.
Sammanfattning
ELISA tillämpades för att studera antigeniteten inom viktiga stammar av Campylobacter samt forekomsten av anti-campylobacter antikroppar hos 394 får och 265 nötkratur. Kanin anti-C. jejuni, C. coli, C. fetus subsp. fetus och C. laridis termo-stabila antigen sera analyse-rades mot 20 Campylobacter stammar och 6 andra bakterier. Anti-C. jejuni och C. coli reagerade starkt med homologa antigener och svagt med antigener av C. fetus subsp. fetus, C. laridis och C. fecalis. C. fetus subsp. fetus serum reagerade främst med sitt homologa antigen. C. laridis serum uppvisade en närmare reaktivitet med C. jejuni an med C. fetus subsp. fetus, C. coli och C. fecalis. Osignifikanta korsreaktioner kunde observeras med termostabila antigener av Y. enterocolitica, S. dublin och E. aerogenes. Tackor vaccinerade med C. fetus subsp. fetus bacterin visade högre ELISA titrar mot C. fetus subsp. fetus antigener än icke vaccinerande tackor eller baggar. 25 % av de vaccinerade djuren hade titrar så låga som 95 % av de icke vaccinerade djuren. Hos nöt konstaterades de lägsta antikroppstitrarna mot C. fetus subsp. fetus, C. jejuni, C. coli och C. laridis antigener i prekolostrala sera, följd av postkolostrala sera och sera från fullvuxna djur. Detta arbete har visat tillämpbarheten av ELISA-testen i seroepidemiologiska undersökningar angående fördelningen och betydelsen av Gampylobacter antikroppar i sera av husdjur för animalie-produktion.
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Gröhn, K., Genigeorgis, C. Adaption of Elisa for the Detection oCampylobacter Antibodies and its Application in Seroepidemiological Studies in Sheep and Cattle Herds. Acta Vet Scand 26, 30–48 (1985). https://doi.org/10.1186/BF03546562
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DOI: https://doi.org/10.1186/BF03546562