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Antibody - Dependent Cell - Mediated Cytotoxicity in Sheep

Antistof - afhængigt celle-medieret cytotoksisitet (ADCC) hos får

Acta Veterinaria Scandinavica volume 26, pages 105–119 (1985)Cite this article

Abstract

The ability of sheep leukocytes to mediate antibody — dependent cell-mediated cytotoxicity (ADCC) and that of sheep serum IgG1 and IgG2 to induce ADCC were investigated. Partial characterization of effector cells was attempted. These investigations revealed that ADCC occurs in sneep. With chicken erythrocytes (CRBC) as the target cells, polymorphonu-cleated cells (PMN), and monocytes, were the most effective leukocytes. Ovine peripheral blood lymphocytes (PBL) also mediated ADCC, and within the PBL population, T-cells were capable of mediating ADCC. The T-cells were obtained by nylon wool fractionation and selective agglutination by peanut agglutinin (PNA) and Helix pomatia agglutinin (HPA). Both nylon wool adherent and non-adherent fractions were active in ADCC, although the former were more active, implying heterogeneity in nylon wool adherence among ovine K-cells. Depletion of B (SIg+) cells did not affect ADCC activity of the remaining cells. Depletion of Fc+ cells markedly reduced cytotoxic activity of PBL. Both sheep IgG1 and IgG2 anti-CRBC immunoglobulins were able to induce ADCC.

Sammendrag

Fåreleukocyters evne til effektuering af ADCC blev undersøgt, ligesom IgG1 og IgG2 undersøgtes for evne til at inducere ADCC. En delvis karakterisering af effektorcellerne forsøgtes. Disse undersøgelser viste, at ADCC forekommer hos får. Med kyllingeerythrocyter som målceller var ovine polymorfnukleære celler, monocyter, de mest effektive celler. Lymfocyter virkede også ved ADCC, og inden for denne population kunne T-celler effektuere ADCC. T-cellerne var isolerede ved nylonvat fraktionering og selektiv agglutinering med PNA og ΗΡΑ. Både på nylonvat adhærerende og ikke-adhærerende fraktioner var aktive i ADCC, skønt de førstnævnte viste størst aktivitet. Dette indicerede heterogenitet for nylonvat adhærans i ovine K-celler. Fjernelse af celler med immun globuliner på cellemembranen påvirkede ikke ADCC aktiviteten af de resterende celler. Såvel IgG1 som IgG2 anti-kyllinge-erytrocyt immunglobuliner kunne forårsage ADCC.

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  1. Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870, Copenhagen V, Denmark

    J. E. D. Mlangwa

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Mlangwa, J.E.D. Antibody - Dependent Cell - Mediated Cytotoxicity in Sheep. Acta Vet Scand 26, 105–119 (1985). https://doi.org/10.1186/BF03546568

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Acta Veterinaria Scandinavica

ISSN: 1751-0147