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A Modified Method for the Detection of Antibiotic Residues in Slaughter Animals

En modifierad metod för påvisande av kemoterapeutika i slaktkroppar.

Acta Veterinaria Scandinavica volume 20, pages 477–491 (1979)Cite this article

Abstract

Biological methods in current use for the detection of antibiotic residues in slaughter animals are reviewed. A modified method is suggested in which the conditions for the control have been standardized. By the use of a semi-defined medium, the batch-to-batch variations are minimized. In order to facilitate the detection of sulfonamides the medium is supplemented with trimethoprim. The standardized conditions included the use of a sporulating organism, Bacillus subtilis, an inoculum size of 0.5 × 105 spores per ml medium, and 5 ml medium of pH 6.0 per plate. A preincubation-diffusion time of 1 h in room temperature is recommended before incubation.

The modified method was compared with the currently prescribed Swedish method. The new method was easier to perform and showed a more uniform sensitivity to most of the antibiotics used.

Sammanfattning

Rester av kemoterapeutika kan förekomma i muskulaturen hos köttproducerande djur efter nutritiv eller terapeutisk behandling med sådana preparat. Vid köttkontrollen används i olika lander olika biologiska metoder för att påvisa rester av kemoterapeutika. Den från 1966 i Sverige föreskrivna metoden uppvisar flera svagheter. En förändrad och bättre standardiserad metod föreslås. Genom användning av ett väldefinierat substrat, Iso-Sensitest agar, och en sporulerande mikroorganism, Bacillus subtilis, uppnås en mindre variation melian dagar och mellan laboratorier. Genom tillsats av trimethoprim förbättras sulfonamidkänsligheten. Lämplig substrat- och inokulationsmängd är 5 ml substrat med 0,5 × 105 sporer per ml. Efter provapplicering förinkuberas plattorna en timme i rumstemperatur. Med den nya metoden kan flera av den tidigare föreskrivna metodens nackdelar undvikas.

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Authors and Affiliations

  1. Department of Food Hygiene, University of Agricultural Sciences, Uppsala, Sweden

    Stefan Fabiansson & Åke Rutegård

  2. Swedish Meat Research Institute, POB 504, S-244 00, Kävlinge, Sweden

    Stefan Fabiansson & Åke Rutegård

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  1. Stefan Fabiansson
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  2. Åke Rutegård
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Fabiansson, S., Rutegård, Å. A Modified Method for the Detection of Antibiotic Residues in Slaughter Animals. Acta Vet Scand 20, 477–491 (1979). https://doi.org/10.1186/BF03546575

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Acta Veterinaria Scandinavica

ISSN: 1751-0147