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Binding of Lipase, Amylase and Protease to Intestinal Epithelium as Affected by Carbohydrates and Lectins in Vitro

Adsorption av tarminnehållets lipas, amylas och proteas till tarmepitelet studerad med hjälp av sockerarter och hemagglutininer in vitro

Acta Veterinaria Scandinavica volume 20, pages 329–342 (1979)Cite this article

Abstract

Intraluminal digestive enzymes were shown to bind to chick intestinal epithelial surface (glycocalyx). Affinity of the intestinal epithelium for the enzymes decreased in the order, lipase > amylase > protease. The plant hemagglutinins, Con A, phytohemagglutinin, pokeweed mitogen and raw soybean effectively released lipase and amylase from the glycocalyx. Based on specific inhibition of binding by sugars, such as fucose and N-acetylated sugars, lipase and amylase appeared to be bound to blood group antigen-like sugar moieties on the glycocalyx of the microvilli.

Sammanfattning

Tarminnehållets pankreashydrolaser adsorberades på ut- och in-vända (inverterade) tarmsegmenter. När segmenterna eluerades i en serie provrör med buffrade koksaltlösningar, frigjordes den proteoly-tiska aktiviteten lättare än den amylolytiska eller lipolytiska aktiviteten. Rangordningen för affiniteten måste därför vara: lipas > amylas > proteas.

För att påvisa, att den epiteliala adsorptionen av pankreashydrolaser är en specifik process, gjordes försök att förhindra adsorptionen eller att frigöra den adsorberade enzymaktiviteten med olika sockerarter (som finns i den normala epiteliala kolhydratsubstansen, glycocalyx). Fucos och N-acetylerade sockerarter frigjorde lipas och amylas aktivitet. Växt hemagglutininer, som reagerar med sockerarter på cellytorna, frigjorde effektivt enzymaktivitetet.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, College of Veterinary Medicine, Helsinki 55, Helsinki, Finland

    M. Sandholm & M. L. Scott

  2. Department of Poultry Science, Cornell University, Ithaca, New York, USA

    M. Sandholm & M. L. Scott

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  1. M. Sandholm
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  2. M. L. Scott
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Sandholm, M., Scott, M.L. Binding of Lipase, Amylase and Protease to Intestinal Epithelium as Affected by Carbohydrates and Lectins in Vitro. Acta Vet Scand 20, 329–342 (1979). https://doi.org/10.1186/BF03546595

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Acta Veterinaria Scandinavica

ISSN: 1751-0147