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A Rapid Radioimmunological Evaluation of the Androstenone Content in Boar Fat

En enkel radioimmunologisk metode for bestemmelse av androstenon i fett fra råner

Acta Veterinaria Scandinavica volume 20, pages 343–350 (1979)Cite this article

Abstract

Fat from boars containing androstenone is absorbed to a piece of filter paper. The filter paper is then placed in a tube and a buffer containing antibodies against androstenone is added. Following incubation the filter paper is removed, and by measurement of the residual binding capacity for androstenone in the buffer a relative value for androstenone content in the fat is obtained.

The coefficient of correlation between the results obtained by this rapid method and the ordinary radioimmunoassay was −0.95.

Sammendrag

Fett fra råner som inneholder androstenon ble absorbert til et stykke filtrerpapir. Filtrerpapiret ble plassert i et reagensglass og en tilsatte antistoffer rettet mot androstenon. Etter at antistoff og steroid hadde bundet seg til hverandre fjernet en filtrerpapiret. Ut fra bestemmelse av resterende bindingskapasitet i antistoffløsningen fikk en et relativt uttrykk for mengde an drosten on i fettet.

Korrelasjonskoeffisienten mellom resultatene en oppnådde med denne enkle metoden og den ordinære radioimmunologiske metoden var −0,95.

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Authors and Affiliations

  1. Department of Reproductive Physiology and Pathology and the Department of Physiology, Veterinary College of Norway, Dep., Oslo 1, P.O.Box 8146, Oslo, Norway

    Øystein Andresen

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  1. Øystein Andresen
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The study was supported by the Norwegian Agricultural Research Council.

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Andresen, Ø. A Rapid Radioimmunological Evaluation of the Androstenone Content in Boar Fat. Acta Vet Scand 20, 343–350 (1979). https://doi.org/10.1186/BF03546596

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Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147