Skip to main content
Download PDF
  • Published:

Relationship Between Production of Enterotoxin and Various Drug Resistance Patterns in Strains of Escherichia Coli Isolated from Piglets Suffering from Colienterotoxaemia

Sammenheng mellom produksjon av enterotoksin og forskjellige resistensmønstre hos stammer av Escherichia coli isolert fra smågris med kolienterotoksemi

Acta Veterinaria Scandinavica volume 20, pages 396–403 (1979)Cite this article

Abstract

The relationship between production of enterotoxin and drug resistance patterns in 92 E. coli strains isolated from the jejunal content from piglets suffering from colienterotoxaemia was investigated. The drugs included were Oxytetracycline, streptomycin, sulphaisodimidin, neomycin, ampicillin and chloramphenicol. The frequency with which transmission of drug resistance occurred as well as the O-groups of these strains were also recorded.

Fifty-one E. coli strains produced enterotoxin. Of these, 42 (approx. 82 %) produced the LT. The same number of strains also produced the ST. Strains simultaneously producing both enterotoxins thus constituted approx. 64 % of the enterotoxin-producing strains. The E. coli strains were divided in 7 groups, each representing different drug resistance patterns. Statistical analysis showed that the various categories of enterotoxin production mentioned above were more frequently found in strains simultaneously resistant to Oxytetracycline, streptomycin and sulphaisodimidin than in strains belonging to the other drug resistance groups in general.

Transmission of drug resistance was demonstrated in all resistance pattern groups. Approx. 50–70 % of the enterotoxin-producing strains showed drug transmission. The O-groups 138 and 149 constituted 8 and 54 out of the 62 typable strains, respectively.

Sammendrag

Forholdet mellom resistens mot antibiotika (oxytetracyklin, streptomycin, sulfaisodimidin, neomycin, ampicillin, chloramphenicol) og produksjon av enterotoksiner ble undersøkt i 92 E. coli-stammer som ble isolert fra tynntarminnhold fra smågriser døde av kolienterotoksemi. Frekvens av overførbar antibiotikaresistens samt stammenes O-grupper ble også undersøkt.

Femtien E. coli-stammer produserte enterotoksin. Av disse produserte 42 (ca. 82 %) LT. Det samme antall stammer produserte ST. Ca. 64 % av de enterotoksinproduserende stammer produserte dermed begge enterotoksiner. E. coli-stammene ble inndelt i 7 ulike grupper hvor hver gruppe representerte et resistensmønster. Ved statistisk beregning viste det seg at de ulike typer av enterotoksinproduksjon som er nevnt ovenfor var hyppigere i stammer som var samtidig resistent mot oxytetracyklin, streptomycin og sulfaisodimidin enn i stammer som hadde et annet resistensmønster.

Overføring av antibiotikaresistens ble påvist innen alle grupper av resistensmønster. I ca. 50–70 % av de enterotoksinproduserende E. coli-stammer ble overførbar antibiotikaresistens påvist. Av de 62 typbare E. coli-stammene tilhørte 8 O-gruppe 138 og 54 O-gruppe 149.

References

  • Dean, A. G., Yi-Chuan Ching, R. G. Williams & L. B. Harden: Test for Escherichia coli enterotoxin using infant mice: Application in a study of diarrhea in children in Honolulu. J. infect. Dis. 1972, 125, 407–411.

    Article  CAS  Google Scholar 

  • Donta, S. T., H. W. Moon & S. C. Whipp: Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science 1974, 183, 334–335.

    Article  CAS  Google Scholar 

  • Kohler, E. M.: Enterotoxic activity of filtrates of Escherichia coli in young pigs. Amer. J. vet. Res. 1968, 29, 2263–2274.

    CAS  PubMed  Google Scholar 

  • Larsen, H. E. & J. L. Larsen: Occurrence and distribution of drug resistant Escherichia coli in feces from cattle and swine. Nord. Vet.-Med. 1972, 24, 651–659.

    CAS  PubMed  Google Scholar 

  • Liven, E.: Drug resistance in strains of Escherichia coli isolated from the intestinal tract of pigs in Norway. Acta vet. scand. 1979, 20, 258–269.

    CAS  PubMed  Google Scholar 

  • Pesti, L. & K. Lukács: Studies on heat stable/ST/enterotoxin produced by Escherichia coli of porcine origin. Proc. 5th Wld. Int. Pig Vet. Soc. Congr., Zagreb 1978.

  • Phillips, R. M. & B. L. Kelly: Identification of enterotoxigenic Escherichia coli isolated from clinical specimens submitted to a diagnostic laboratory. Proc, 5th Wld Int. Pig Vet. Soc. Congr., Zagreb 1978.

  • Sack, R. B.: Human diarrheal disease caused by enterotoxigenic Escherichia coli. Ann. Rev. Microbiol. 1975, 29, 333–353.

    Article  CAS  Google Scholar 

  • Sack, D. A. & R. B. Sack: Test for enterotoxigenic Escherichia coli using Y1 adrenal cells in miniculture. Infect. Immun. 1974, 11, 334–336.

    Article  Google Scholar 

  • Sack, R. B., S. L. Gorbach, J. G. Banwell, B. Jacobs, B. D. Chatterjee & R. C. Mitra: Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease. J. infect. Dis. 1971, 123, 378–385.

    Article  CAS  Google Scholar 

  • Skovgaard, N.: Tarmpatogene mikroorganismer og matforgiftning, en oversikt. (Enteropathogenic organisms and food poisoning, a survey). Proc, 13th Nord. Vet. Congr., Åbo 1978.

  • Smith, H. W. & S. Halls: Observation on infective drug resistance in Britain. Brit. med. J. 1966, 1, 266–269.

    Article  CAS  Google Scholar 

  • Smith, H. W. & S. Halls: Studies on Escherichia coli enterotoxin. J. Path. Bact. 1967, 93, 531–543.

    Article  CAS  Google Scholar 

  • Smith, H. W. & S. Halls: The transmissible nature of the genetic factor in Escherichia coli that controls enterotoxin production. J. gen. Microbiol. 1968, 52, 319–334.

    Article  Google Scholar 

  • Søderlind, O., T. Wadstrøm & R. Møllby: Colibacillosis in pigs. Proc, 4th Int. Pig Vet. Soc. Congr., Ames 1976.

  • Tschäpe, H. & H. Rische: Die Virulenz-Plasmide der Enterobacteriaceae. (The virulens-plasmids of Enterobacteriaceae). Z. allg. Mikrobiol. 1974, 14, 337–350.

    Article  Google Scholar 

  • Walton, J. R.: The relationship between the transferability of enterotoxin plasmids and the simultaneous transfer of antibiotic resistance plasmids. Zbl. Vet.-Med. B 1977, 24, 317–324.

    Article  CAS  Google Scholar 

  • Wierup, M.: Antibiotic resistance and transferable antibiotic resistance of Escherichia coli isolated from Swedish calves 5 and 30 days old. Nord. Vet.-Med. 1975, 27, 77–84.

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Veterinary College of Norway, Dep, Oslo 1, Post-box 8146, Oslo, Norway

    Eivind Liven

Authors
  1. Eivind Liven
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The investigation was made possible by financial support from the Agricultural Research Council of Norway.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liven, E. Relationship Between Production of Enterotoxin and Various Drug Resistance Patterns in Strains of Escherichia Coli Isolated from Piglets Suffering from Colienterotoxaemia. Acta Vet Scand 20, 396–403 (1979). https://doi.org/10.1186/BF03546601

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03546601

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147