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The Characterization of Equine Prealbumin (Pr) Proteins by Antigen-Antibody Crossed Electrophoresis

Prealbumin (Pr) proteiner i hesteserum karakterisert ved antigenantistoff krysselektroforese

Acta Veterinaria Scandinavica volume 20, pages 180–190 (1979)Cite this article

Abstract

The characterization of equine prealbumin (Pr) proteins by antigen-antibody crossed electrophoresis. Acta vet. scand. 1979, 20, 180–190. — Selected equine Pr phenotypes from a total of 55 horses of mixed breeds were investigated. The horse sera were subjected to acid starch gel electrophoresis at pH 4.8, followed by right angle electrophoresis in agarose gels containing rabbit-produced anti-Pr protein. This technique gives peaks in the agarose gels corresponding to the Pr zones in acid gels.

The investigation revealed patterns of the Pr protein which were more complex than those seen when using ordinary acid starch gel electrophoresis. The phenotypes FF, II and LL showed a total of eight peaks, each with three main peaks in the front. Ahead of these, the Pr II and Pr LL phenotypes each had a fourth small peak. The basic fast pattern for these two phenotypes therefore consisted of four bands. The Pr WW and Pr SS showed a similar picture as regards the fast moving peaks. The Pr NN type appeared with two peaks in the front, one small and one large and with two slow moving ones. The Pr UU type had four peaks, but only in the area of the main Pr U band in acid gels.

Four heterozygous Pr phenotypes appeared as a combination of the corresponding homozygous phenotypes, the number and height of the peaks depending on positions and overlappings of these in the respective homozygotes. Thus the Pr FW phenotype showed a total of 10 peaks.

The effect of variations in pH of the starch gel buffer was studied. The Pr NN and Pr FF phenotypes were run at pH 4.8, 5.0, 5.2 and 5.4. With increasing pH, the slow moving peaks weakened and moved closer to the fast ones. At pH 5.4 only one large fast moving peak remained.

Sammendrag

Det ble foretatt en undersøkelse av besternte Pr fenotyper has hest på et totalantall av 55 hester av forskj eilige raser. Hestesera ble kjørt på stivelseselektroforese ved pH 4.8 etterfulgt av elektroforese i rett vinkel på agarose gel som inneholdt anti-Pr-protein produsert på kanin.

Undersøkelsen åpenbarte mere komplekse mønstre av Pr proteinet enn det som er sett ved ordinær elektroforese på stivelsesgel. Fenotypene FF, II og LL viste et totalantall på åtte topper hver med tre hovedtopper fairest. Foran disse hadde Pr II og Pr LL fenotypene hver en fjerde mindre topp. Det fremre hovedmønster for disse to fenotyper besto derfor av fire band.

Pr WW og Pr SS hadde et lignende mønster når det gjaldt de hurtige topper. Pr NN typen viste to topper forrest, en liten og en stor, og i tillegg to mere langsomtgàende topper. Pr UU typen hadde fire topper uten å ha noen som vandret långsomt.

Fire heterozygote Pr fenotyper viste seg som en kombinasjon av de tilsvarende homozygote fenotyper, idet antall og høyder av topper avhang av posisjoner og overlappinger av toppene i de respektive homozygoter. Således viste Pr FW fenotypen et totalantall på ti topper.

Virkningene av variasjoner i pH i stivelsesgelbufferen ble undersøkt. Pr NN og Pr FF fenotype ble kjørt ved pH 4,8, 5,0, 5,2 og 5,4. Ved stigende pH ble de saktegående topper mindre og bevæget seg nærmere de hurtige. Ved pH 5,4 gjensto bare en hurtiggående topp.

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  1. Department of Internal Medicine I, Veterinary College of Norway, Dep., Oslo 1, P. O. Rox 8146, Oslo, Norway

    Nils Ek

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Ek, N. The Characterization of Equine Prealbumin (Pr) Proteins by Antigen-Antibody Crossed Electrophoresis. Acta Vet Scand 20, 180–190 (1979). https://doi.org/10.1186/BF03546610

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Acta Veterinaria Scandinavica

ISSN: 1751-0147