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An Improved Microbiological Procedure for Detection of Streptomycin Residues in Milk and Animal Tissues

En forbedret mikrobiologisk metode til påvisning af streptomycinrester i mælk og animait væu

Acta Veterinaria Scandinavica volume 20, pages 38–44 (1979)Cite this article

Abstract

A method is described which allows detection of 0.025 µg streptomycin sulfate per ml. This represents an improvement of sensitivity by 8 times when compared with the currently used method. By adding penicillin to the assay medium in subinhibitory concentrations, a synergistic effect of streptomycin and penicillin is exerted towards the test organism, Bacillus subtilis, resulting in an increased sensitivity to streptomycin.

Sammendrag

Der beskrives en metode, der tillader påvisning af 0,025 µg streptomycin sulfat pr. ml. Dette er en forbedring af følsomheden på 8 gange sammenlignet med den for tiden anvendte metode. Ved at sætte penicillin til substratet i subinhibitive koncentrationer opnås en synergistisk virkning af streptomycin og penicillin over for testorganismen, Bacillus subtilis, hvilket resulterer i en forøget følsomhed for streptomycin.

References

  • Fabiansson, S., Å. Rutegård, R. Sueide & H. Bremer: Försök med en ny metod för kontroll av kemoterapeutika i slaktkroppar. (Experiments with a new method for control of chemotherapeutic agents in slaughtered animals). Svensk Vet.-Tidn. 1977, 29, 953–965.

    Google Scholar 

  • Gudding, R.: An improved bacteriological method for the detection of sulfonamide residues in food. Acta vet. scand. 1976, 17, 458–464.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Inglis, Jean H. & S. E. Katz: Improved microbiological assay procedures for dihydrostreptomycin residues in milk and dairy products. Appl. env. Microbiol. 1978, 35, 517–520.

    Article  CAS  Google Scholar 

  • Jawetz, E., J. B. Gunnison & V. R. Coleman: The combined action of penicillin with streptomycin or Chloromycetin on enterococci in vitro. Science 1950, 111 254–256.

  • Kornfeld, J. M.: Enhancement of penicillin-induced lysis in Sarcina lutea by lysozyme. J. Food Prot. 1977, 40, 555–557.

    Article  PubMed  Google Scholar 

  • Moellering, R. C. & A. N. Weinberg: Studies on antibiotic synergism against enterococci. II. Effect of various antibiotics on the uptake of 14C-labelled streptomycin by enterococci. J. clin. Invest. 1971, 50, 2580–2584.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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  1. Odense Municipal Laboratory, Lille Tornbjerg Vej 24, DK-5260, Odense, SØ, Denmark

    Henry Søgaard

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  1. Henry Søgaard
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Søgaard, H. An Improved Microbiological Procedure for Detection of Streptomycin Residues in Milk and Animal Tissues. Acta Vet Scand 20, 38–44 (1979). https://doi.org/10.1186/BF03546627

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  • DOI: https://doi.org/10.1186/BF03546627

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147