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Glutathione Peroxidase Activity and Erythrocyte Lipid Peroxidation as Indices of Selenium and Vitamin E Status in Young Pigs

Glutation-peroxydase-aktivitet og erytrocyt-lipid-peroxydering som mål for seien- og vitamin E-status hos unge grise

Acta Veterinaria Scandinavica volume 20, pages 92–101 (1979)Cite this article

Abstract

A randomized, blocked 23 factorial experiment was conducted with 48 pigs from sows fed a diet low in selenium and vitamin E. From 3 to 12 weeks of age the piglets were kept in single pens and fed a basic diet consisting mostly of barley, dried skim milk, soybean meal and dried yeast, and containing 55 µg selenium and 3 mg vitamin E per kg. The treatment factors — i.e. feed supplements — were 2 levels of Se (nil, 60 µg/kg), 2 levels of vitamin E (nil, 50 mg/kg), and 2 levels of the feed antioxidant ethoxyquin (nil, 150 mg/kg). Blood samples, collected at termination of the experiment, were examined for glutathione peroxidase activity (GSH-Px) and resistance against erythrocyte lipid peroxidation (ELP) to evaluate Se and vitamin E status, respectively. Analysis of variance showed the GSH-Px activity to be litter-dependent (P < 0.001) and influenced by selenium supplementation (P < 0.001) but not by the other supplements or by interactions between supplements. Resistance against ELP was influenced only by vitamin E supplementation (P < 0.001). GSH-Px and ELP thus seem to be valuable and simple methods for evaluating, respectively, Se status and vitamin E status in growing pigs.

Sammendrag

I et 23 faktorielt fodringsforsøg med tilfældigt sammensatte blokke blev anvendt grise fra søer, der var fodret med et foder fattigt på seien og vitamin E. Fra grisene var tre uger og til de blev tolv uger garnie, blev de fodret i enkeltdyrsstier med et grundfoder, der hovedsagelig bestod af byg, tørret skummetmælk, soyaskrå og tørgaer, og som indeholdt 55 µg seien og 3 mg vitamin E pr. kg. Behandlingsfaktorerne var forskellige fodertilskud, hver i to niveauer: seien (0 og 60 µg/kg), vitamin E (0 og 50 mg/kg) og foder-antioxydanten ethoxyquin (0 og 150 mg/kg). Blodprøver, der blev udtaget ved forsøgets afslutning, blev undersøgt for glutation-peroxydase-aktivitet (GSH-Px) og resistens mod erytrocyt-lipid-peroxydering (ELP) med henblik på en vurdering af henholdsvis seien og vitamin E status.

Ved en variansanalyse fandtes GSH-Px at vsere afhsengig af selentilskuddet (P < 0.001) og af kuld (P < 0.001), men ikke af de andre fodertilskud eller af samspil mellem forskellige fodertilskud. Resistens mod ELP var kun afhængig af vitamin E tilskuddet (P < 0.001). Vurderet ud fra denne undersøgelse synes GSH-Px og ELP at kunne være værdifulde simple metoder til en uafhængig vurdering af seien status og vitamin E status hos unge grise.

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Authors and Affiliations

  1. State Veterinary Serum Laboratory and the Department for Experiments with Pigs and Horses, National Institute of Animal Science, Bülowsvej 27, Copenhagen V, DK-1870, Copenhagen, Denmark

    P. Thode Jensen, V. Danielsen & H. E. Nielsen

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  1. P. Thode Jensen
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  2. V. Danielsen
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  3. H. E. Nielsen
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This study was supported by a grant from the Danish Agricultural and Veterinary Research Council.

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Jensen, P.T., Danielsen, V. & Nielsen, H.E. Glutathione Peroxidase Activity and Erythrocyte Lipid Peroxidation as Indices of Selenium and Vitamin E Status in Young Pigs. Acta Vet Scand 20, 92–101 (1979). https://doi.org/10.1186/BF03546633

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Acta Veterinaria Scandinavica

ISSN: 1751-0147