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The Demonstration and Characterization of Deoxyribonucleases of Streptococci Group A, B, C, G And L

PÃ¥visning og karakterisering au deoxyribonuklease produsert av streptokokker gruppe A, B, C, G og L

Acta Veterinaria Scandinavica volume 20, pages 102–121 (1979)Cite this article

Abstract

Streptococcus pyogenes, S. agalactiae, S. dysgalactiae, S. equi, S. equisimilis, S. zooepidemicus, Streptococcus group G and L were found to produce deoxyribonucleases (DNases) which were demonstrated using the Toluidine Blue DNA Agar (TDA) described for staphylococcal DNases. The activity of streptococcal DNases increased in the presence of Mg++ and Ca++ ions, the pH optimum was about 7.5 and native DNA was the best enzyme substrate. It is consequently recommended to modify the TDA according to these results for the demonstration of streptococcal DNases. All streptococcal DNases, except the DNase of S. zooepidemicus, were found to be heat-stable. Isoelectric focusing was a convenient technique for separation of streptococcal DNases and for estimation of the pI values of the DNases. S. agalactiae and S. dysgalactiae generally exhibited distinct species specific patterns in the isoelectric focusing experiments. The DNases produced by S. pyogenes were serologically related to the DNases of S. dysgalactiae and Streptococcus group G. A similar relationship was demonstrated between the DNases produced by S. equisimilis and Streptococcus group L.

Sammendrag

Streptococcus pyogenes, S. agalactiae, S. dysgalactiae, S. equi, S. equisimilis, S. zooepidemicus, Streptococcus gruppe G og L produserte deoxyribonukleaser (DNaser) som ble påvist ved bruk av toluidinblått -DNA-agar. Aktiviteten av disse DNasene økte i nærvaer av M++- og Ga++-ioner, pH-optimum var ca. 7,5, og nativ DNA var det beste enzymsubstratet. Ved undersøkelse av DNaser produsert av streptokokker bør sammensetningen av TDA endres i samsvar med disse resultatene. DNaser fra alle streptokokkene, unntatt S. zooepidemicus, var varmestabile. Isoelektrisk fokusering av DNasene var en hensiktsmessig metode for separering av disse enzymene og for å anslå pI-verdiene. DNasene fra S. pyogenes var serologisk beslektet med DNasene fra S. dysgalactiae og Streptococcus gruppe G. Et lignende slektskap ble påvist mellom DNasene fra Streptococcus equisimilis og Streptococcus gruppe L.

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  1. National Veterinary Institute, Dep., Oslo 1, Postbox 8156, Oslo, Norway

    Roar Gudding

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  1. Roar Gudding
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Gudding, R. The Demonstration and Characterization of Deoxyribonucleases of Streptococci Group A, B, C, G And L. Acta Vet Scand 20, 102–121 (1979). https://doi.org/10.1186/BF03546634

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Acta Veterinaria Scandinavica

ISSN: 1751-0147