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Agarose Gel Electrophoretic Separation of Blood Serum Proteins in Cattle
Agarosgelelektrofores au blodserumproteiner hos nötkreatur
Acta Veterinaria Scandinavica volume 16, pages 520–524 (1975)
Abstract
The agarose gel electrophoresis described by Johansson (1972) was modified so that a buffer of pH 7.9 was used in the gel, whereas the buffer in the electrode vessels had a pH of 8.6.
The cattle blood serum protein picture is described in detail. The β1-globulin zone shows a very distinct picture of the genetically polymorphic bovine transferrins. The region between the α- and β-globulins shows a number of faint and often very distinct bands. A faint background staining over the whole electrophoretogram may partly be caused by a rather strong lipoprotein in the α1-region, lipids thus having migrated all over the electrophoretogram.
The modified method described is well suited as a “screen electrophoresis” for cattle serum and is also useful e.g. in studying bovine transferrin polymorphism.
Sammanfattning
Den agarosgelelektrofores som beskrivits av Johansson (1972) har modifierais så att buffert med pH 7,9 användes i gelet, medan bufferten i elektrodkärlen är av pH 8,6. Med denna och några andra, smärre modifikationer har metoden blivit lämplig för “screen”-elektroforetiska undersökningar av blod från nötkreatur.
Den serumproteinbild som erhålles hos nötkreatur med denna metod beskrivs i detalj. β1-globulin-zonen uppvisar en mycket distinkt bild av transferrinpolymorfismen hos nötkreatur. Området mellan α- och β-globulin visar ett antal svaga men ofta mycket distinkta proteinband. En svag bakgrundsfärgning över hela elektroforetogrammet kan möjligen till viss del orsakas av ett ganska starkt α1-lipoprotein, där sälunda lipoider migrerat hela vägen över elektroforetogrammet.
References
Aalund, O.: Immune response of sheep, goats, cattle and swine. In Immunity to Animal Parasites. Acad. Press, Inc., New York and London 1972.
Johansson, B. G.: Agarose gel electrophoresis. In C.-R. Laurell: Electrophoretic and Electro-Imimuno-Ghemical Analysis of Proteins. Seand, J. clin. Lab. Invest. 1972, 29, Suppl. 124.
Laurell, C.-B.: Quantitative estimations by electrophoresis in agarose gel containing antibodies. Analyt. Riochem. 1966, 15, 45–52.
Murphy, F. A., J. W. Osebold & O. Aalund: Physical heterogeneity of bovine γ-globulins: Characterization of γM and γG globulins. Arch. Riochem. 1965, 112, 126–136.
Wieme, R. J.: Studies on agar gel electrophoresis. (Arcia Uitgaven N. V. Rrussels; and Elsevier, Amsterdam 1965). 1959.
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The investigations were supported by the Swedish Council for Forestry and Agricultural Research.
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Carlström, G., Liberg, P. Agarose Gel Electrophoretic Separation of Blood Serum Proteins in Cattle. Acta Vet Scand 16, 520–524 (1975). https://doi.org/10.1186/BF03546645
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DOI: https://doi.org/10.1186/BF03546645