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Enzyme Histochemical Studies of the Normal Pig Kidney

Enzymhistokemiske undersøgelser af den normale grisenyre

Acta Veterinaria Scandinavica volume 16, pages 153–162 (1975)Cite this article

Abstract

The activity and localization of NAD(P)H-tetrazolium reductase, lactate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, glueose-6-phosphate dehydrogenase, α-glycerophosphate dehydrogenase, acid phosphatase, and alkaline phosphatase in the kidney of 11 female pigs were examined.

The pig kidney showed a higher activity of NAD (P) H-tetrazolium reductase in the distal tubules compared with the kidney of rat, mouse, rabbit, dog, cat, and man. The activity of succinate dehydrogenase and alkaline phosphatase was the same in the pig kidney as in the kidney of other examined species. In the pig kidney glucose-6-phosphate dehydrogenase precipitated in situ, while in rat and mouse this enzyme has proved to be highly diffusible.

Sammendrag

Aktiviteten og lokalisationen af NAD(P)H-tetrazolium reduktase, mælkesyredehydrogenase, isocitronsyredehydrogenase, ravsyredehydrogenase, glukose-6-fosfatdehydrogenase, α-glycerofosfatdehydrogen-ase, samt sur og alkalisk fosfatase blev undersøgt på 11 hungrise. Grisenyrens enzymprofil blev sammenlignet med andre pattedyrs. For NAD(P)H-tetrazolium reduktasens vedkommende viste det sig, at de distale tubulusafsnit i grisenyren viste en højere aktivitet end de samme afsnit i nyren hos rotte, mus, kanin, hund, kat og menneske. Der var god overensstemmelse mellem aktiviteten af ravsyredehydrogenase og alkalisk fosfatase i grisenyren sammenlignet med nyren fra andre undersøgte species. Ved reaktionen for glucose-6-fosfatdehydrogenasen fældede reaktionsproduktet ud in situ, medens dette enzym har vist en kraftig tendens til at diffundere hos mus og rotte.

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Authors and Affiliations

  1. Department of Pathology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Copenhagen V, Denmark

    F. Elling & T. Møller

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  1. F. Elling
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  2. T. Møller
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This study was supported by the Danish Agricultural and Veterinary Research Council.

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Elling, F., Møller, T. Enzyme Histochemical Studies of the Normal Pig Kidney. Acta Vet Scand 16, 153–162 (1975). https://doi.org/10.1186/BF03546669

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Acta Veterinaria Scandinavica

ISSN: 1751-0147