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The Value of the Glutaraldehyde and Formaldehyde Tests in Evaluation of the Globulin Level in Bovine Blood
Glutaraldehydtest och formaldehydtest för bestämning au globulinstatus i bovint blod
Acta Veterinaria Scandinavica volume 16, pages 236–243 (1975)
Abstract
So-called liability reactions are based on the gelification of blood globulins when reacting with, i.e., glutaraldehyde or formaldehyde. Solidifying time is inversely proportional to the globulin concentration of the blood. In the present investigation glutaraldehyde and formaline-gelification tests were compared concerning their diagnostic and prognostic value in dairy cows with internal diseases.
The investigation confirmed earlier results. The correlation between globulin concentration and gelification time was significant, and both tests give very good possibilities to reveal hyper-γ-globulinaemias.
The 2 tests were relatively equal as regards the reliability. Even though the formol-gel reaction allows a somewhat better safety and semi-quantitative differentiation than the glutaraldehyde test, the latter has obvious practical advantages. It can be done on whole blood and with a short observation time. Thus, the blood globulin status can be evaluated during the visit on the farm.
Sammanfattning
Så kallade labilitetsreaktioner bygger på att blodets globuliner vid reaktion med t. ex. glutaraldehyd eller formaldehyd omvandlas till gel. Stelningstiden är omvänt proportioneil till blodets globulinkoncentration.
I föreliggande arbete jämfördes glutaraldehydtester och formolgelreaktioner, med speciell inriktning på testernas diagnostiska och prognostiska värde vid internmedicinska sjukdomar hos mjölkkor.
Undersökningen bekräftade tidigare resultat. Korrelationen mellan globulinkoncentrationen och gelbildningstid ger för båda testerna mycket goda möjligheter alt avslöja hyperglobulinemier.
Testerna visade tämligen likvärdig tillförlitlighet. Även om formolgelreaktionen ger möjlighet till nägot större semikvantitativ differentiering och säkerhet än glutaraldehydtestet har det sistnämnda uppenbara praktiska fördelar. Det kan användas på helblod och kräver endast kort observationstid. Blodglobulinstatus kan därför fastställas redan vid besöket på gården.
References
Doumas, B. T., W. A. Watson & H. G. Biggs: Albumin standards and the measurement of serum albumin with bromcresol green. Glin. chim. Acta 1971, 31, 87–96.
Jönsson, G. & P. Liberg: Liver abscesses in intensively fed cattle. Serial investigations of serum proteins. Acta vet. scand. 1974, 15, 264–273.
Liberg, P.: The formol-gel reaction in cattle. Acta vet. scand. 1973, 14, 712–722.
Sandholm, M.: A preliminary report of a rapid method for the demonstration of abnormal gammaglobulin levels in bovine whole blood. Res. vet. Sci. 1974, 17, 32–35.
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The investigation was supported by the Swedish Council for Forestry and Agricultural Research.
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Liberg, P., Pehrson, B. & Sandholm, M. The Value of the Glutaraldehyde and Formaldehyde Tests in Evaluation of the Globulin Level in Bovine Blood. Acta Vet Scand 16, 236–243 (1975). https://doi.org/10.1186/BF03546678
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DOI: https://doi.org/10.1186/BF03546678