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Immunochemical Quantitation of Pig Transferrin by Single Radial Immunodiffusion
Immimokemisk bestemmelse af transferrin hos svin ved enkelt radial immunodiffusion
Acta Veterinaria Scandinavica volume 16, pages 244–250 (1975)
Abstract
A system has been developed for immunochemical determination of pig transferrin. Direct comparison of immunological values with chemical values showed that monovalent rabbit anti-pig serum transferrin is suitable for the immunological estimation of iron binding protein in swine.
Sammendrag
Der er udviklet et system til immunokemisk bestemmelse af transferrin. Ved sammenligning af resultater fra immunologiske og kemiske bestemmelser fandtes, at monovalent kanin anti-svine-serum-transferrin er egnet til immunologisk bestemmelse af jernbindende protein i svineserum.
References
Bothwell, T. H. & C. A. Finch: Iron Metabolism. Publ. J. & A. Churchill Ltd., London 1962.
Eckman, I., J. B. Robbins, C. J. A. Van den Homer, J. Lentz & I. H. Scheinberg: Automation of a quantitative immunochemical microanalysis of human serum transferrin: A model system. Clin. Chem. 1970, 16, 558–561.
Fischl, J. & S. Cohen: A simple colorimetric method for the determination of total iron binding capacity of serum. Clin. chim. Acta 1962, 7, 121–123.
Goodman, M., H. S. Newman & D. S. Ramsey: The use of chicken antiserum for the rapid determination of plasma protein components. 3. The assay of human serum transferrin. J. Lab. clin. Med. 1958, 51, 814–823.
Hristić, V.: Transferini i metabolizam gvozdja u svinja. (Transferrins and iron metabolism in swine). Thesis. Beograd 1971.
Hristić, V. & M. Movsesijan: Isolation of transferrin from pig serum. Acta vet. (Beogr.) 1974, 24, 95–102.
Jager, B. V. & C. J. Gubler: An immunologic study of the iron-binding protein. J. Immunol. 1952, 69, 311–318.
Jensen, K. B.: Immunochemical determination of serum concentrations of albumin, IgG and IgM. Protides biol. Fluids 1966, 14, 677–681.
Laurell, C. B. & B. Ingelman: The iron-binding protein of swine serum. Acta chem. scand. 1947, 1, 770–776.
Lowry, O. H., N. J. Rosebrough, A. L. Farr & R. J. Randall: Protein measurement with Folin phenol reagent. J. biol. Chem. 1951, 193, 265–275.
Mancini, G., J.-P. Vaerman, A. O. Carbonara & J. F. Heremans: A single-radial-diffusion method for the immunological quantitation of proteins. Protides biol. Fluids 1963, 11, 370–373.
Mancini, G., A. O. Carbonara & J. F. Heremans: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 1965, 2, 235–254.
Ouchterlony, Ö.: Diffusion-in-gel methods for immunological analysis. Progr. Allergy 1958, 5, 1–78.
Scheidegger, J. J.: Une micro-méthode de l’immuno-électrophorèse. (A micromethod of immuno-electrophoresis). Int. Arch. Allergy 1955, 7, 103–110.
Wegger, I., U. Westergaard, G. Kovács & V. Hristić: Svinets transferriner og disses jernbindende evne. (Total iron binding capacity in pigs). Kgl. Vet.- og Landbohøjsk., Inst. Sterilitetsforskn. Aarsberetn. 1964, 147–153. Copenhagen.
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Hristić, V., Movsesijan, M. Immunochemical Quantitation of Pig Transferrin by Single Radial Immunodiffusion. Acta Vet Scand 16, 244–250 (1975). https://doi.org/10.1186/BF03546679
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DOI: https://doi.org/10.1186/BF03546679