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Species Specificity in Thermostable and Ethanol Insoluble Tissue Antigens
Artsspesifisitet i termostabile og etanoluløselige vevsantigener. I. Immunisering av kanin og geit med storfeantigen
I. Immunization of Rabbits and Goats with Bovine Antigen
Acta Veterinaria Scandinavica volume 16, pages 251–257 (1975)
Abstract
Kidney and spleen antigens from cow, sheep, goat, European moose, reindeer, deer and roe deer were prepared by boiling and ethanol precipitation and tested against rabbit and caprine anti-bovine kidney sera. Two different levels of antigen concentration were used for immunizing animals in the various groups. In the group using high antigen concentrations, the precipitation reaction obtained initially disappeared after further inoculations, probably due to immunological tolerance. Sera tested from animals inoculated with low level antigen concentration showed a variation in the immunological response. The caprine antibovine sera showed distinct species specific reaction, while rabbit antisera showed either species specific or organ specific reaction, with a limited degree of cross reaction. In the production of species specific antisera against ruminant tissue antigens, goat seems to be preferable to rabbit.
Sammendrag
Nyre- og miltantigen fra storfe, sau, geit, elg, reinsdyr, hjort og rådyr ble preparert ved koking og alkoholpresipitering og testet mot antistorfenyresera fra kaniner og geiter. To ulike antigenkonsentrasjoner ble brukt ved immuniseringen av dyregruppene. I gruppen som ble podet med høy antigenkonsentrasjon, ble det først observert presipitasjonsreaksjon, men den ne forsvant etter siste poding, sannsynligvis p.g.a. immunologisk toleranse. Sera fra dyrene som ble podet med lav dose antigen viste forskjeilig immunrespons. Antistorfesera fra geit ga tydlig artsspesifikk reaksjon, mens antistorfesera fra kanin viste enten artsspesifikk eller organspesifikk reaksjon og i noen grad kryssreaksjon. Ved produksjon av antisera mot vevsantigen fra drøvtyggere synes geit å være bedre egnet enn kanin.
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Andersen, A.E. Species Specificity in Thermostable and Ethanol Insoluble Tissue Antigens. Acta Vet Scand 16, 251–257 (1975). https://doi.org/10.1186/BF03546680
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DOI: https://doi.org/10.1186/BF03546680