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Cobalt Metabolism in Horse

Koboltmetabolismen hos häst. Koncentration i serum och biosyntes av vitamin B12

Serum Level and Biosynthesis of Vitamin B12

Acta Veterinaria Scandinavica volume 16, pages 84–94 (1975)Cite this article

Abstract

The levels of serum vitamin B12 were determined on 16 mature partly warm-blooded, partly Finnish rural-race horses by the radioisotopic competitive inhibition assay method. The mean value from three samplings carried out in dupli- or triplicate was 1.54 ± 0.16 ng/ml. The utilization of serum inorganic cobalt for cyanocobalamin synthesis was studied on two geldings, which received a dose of 200 µCi 58CoGl2 i.v. A Sephadex G-100 gel filtration was carried out with the serum proteins from serial blood samplings at different time intervals 15 min. to 48 hrs. after administration. The gel filtration showed the presence of two labelled proteins in the serum, one of them appearing some time after administration and disappearing almost completely towards the end of the experimental period. The two elution peaks are considered to represent inorganic 58Co and 58Co labelled vitamin B12. The appearance of labelling in serum vitamin B12 indicates the passing of cobalt into the intestine, and reabsorption into blood in the form of vitamin B12.

Sammanfattning

Koncentrationen av vitamin B12 i serum bestämdes hos 16 vuxna delvis varmbio diga, delvis finska hästar med hjälp av den kompetitiva radioisotop-inhibitionsmetoden. Medelvärdet av tre dubbelt eller tredubbelt undersökta prov var 1,54 ± 0,16 ng/ml. Utnyttjandet av serum oorganiskt kobolt för biosyntes av cyanokobalamin undersöktes hos två vallacker, som fick en dos av 200 µCi 58CoCl2 intravenöst. En Sephadex G-100 gelfiltration utfördes med serumproteiner från en serie av blodprov tagna 15 min. till 48 timmar efter injektionen. Gelfiltrationen visade, att det fanns två märkta proteiner i serum varvid den ena av dessa uppkom först en tid efter injektionen och försvann nästan helt mot slutet av försöksperioden. De två elutionstopparna ansågs representera oorganiskt 58Go och med 58Go märkt vitamin B12. Uppkomsten av märkning serum vitamin B12 visar, att kobolt har passerat in i tarminnehållet, och reabsorberat i form av vitamin B12.

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  1. Department of Biochemistry, Gollege of Veterinary Medicine, Helsinki, Finland

    K. Salminen

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  1. K. Salminen
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Salminen, K. Cobalt Metabolism in Horse. Acta Vet Scand 16, 84–94 (1975). https://doi.org/10.1186/BF03546698

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Acta Veterinaria Scandinavica

ISSN: 1751-0147