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Plaque Assay of Bovine Rotavirus

Plaque titrering af bovin rotavirus

Acta Veterinaria Scandinavica volume 24, pages 362–373 (1983)Cite this article

Abstract

Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.

Sammendrag

Ved at lade trypsin og diethylaminoethyl-dextran indgå i det faste substrat var det muligt at påvise bovin rotavirus (UK stammen) gennem plaque teknik. I kulturer af abeceller (BGM celler) blev der op-nået 1 mm store plaques, mens der i sekundaere kulturer af kalvenyre-celler regelmaessigt sås plaque af 3—4 mm st0rrelse.

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Authors and Affiliations

  1. Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870, Copenhagen V, Denmark

    G. Butchaiah & E. Lund

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  1. G. Butchaiah
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  2. E. Lund
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Butchaiah, G., Lund, E. Plaque Assay of Bovine Rotavirus. Acta Vet Scand 24, 362–373 (1983). https://doi.org/10.1186/BF03546710

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Acta Veterinaria Scandinavica

ISSN: 1751-0147