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Plasma Luteinizing Hormone Response to Increasing doses of Synthetic Gonadotrophin-Releasing Hormone in Heifers

Plasma luteiniserende hormon respons hos kvier efter stigende doser af syntetisk gonadotropin-releasing hormon

Acta Veterinaria Scandinavica volume 24, pages 211–224 (1983)Cite this article

Abstract

The dosenresponse relationship for a synthetic gonadotrophin-releasing hormone (GnRH) was studied in normally cycling heifers using the area under the luteinizing hormone (LH) curve as a response parameter. Oestrus was synchronized by an injection of 0.5 mg cloprostenol before the experiment started and after the 3rd treatment with GnRH. Treatment with GnRH as assigned in a Latin square included 5 dose levels (0, 10, 50, 100, 250 μg) and 5 treatment days over a period of 22 days. GnRH was capable of inducing an increase of plasma LH within 30 min after injection. Plasma LH response increased with increasing doses of GnRH, the largest increase being observed when the dose was raised from 50 μg to 100 μg. One heifer did not respond to any of the doses applied. The existence of an individual treshold dose of GnRH is suggested.

Sammendrag

Det syntetiske gonadotropin-releasing hormón (GnRH, Nialutin®) anvendt i dette forsøg er af identisk struktur med det naturlige releasinghormon i hypothalamus og påvirker hypofyseforlappen til udskillelse af gonadotropiner. GnRH har været anvendt til at indurare luteinisering af follikler, til igangsætning af østralcyklus hos køer med follikelcyster og til at fremkalde ovulation hos køer i den tidlige postpartum période. Dosisstørrelse liar vseret meget varierende, og behand-lingseffekten har oftest vseret vurderet på basis af en klinisk under-søgelse sammenholdt med gonadotropin-udskillelsen, bl. a. mølt som den maksimale udskillelse af luteiniserende hormón (LH toppen) og varigheden af denne top. Et andet og måske mere korrekt biologisk mål for LH respons er arealet under LH kurven, og formålet med dette forsøg var at studere GnRH dosisrespons for stigende doser GnRH ved at benytte en arealberegning som responsparame ter.

GnRH behandlingerne blev foretaget såvel i proøstrus som i diøstrus, da tidligere forsøg har vist at follikelcyster ofte indeholder luteinvæv. I forsøget indgik 5 kvier med normal østralcyklus. Cyklus blev synkroniseret med 0,5 mg cloprostenol (Estrumate®). Behandling med 0, 10, 50, 100 og 250 μg GnRH blev foretaget på 5 forsøgsdage fordelt over en periode på 22 dage. GnRH injektion medførte en stig-ning i plasma-LH i løbet af 30 min og LH niveauet forblev højt i 4–5½ time. Plasma-LH responset, malt som arealet under LH kurven, steg med stigende dosis GnRH. Den største stigning fandtes, når dosis steg fra 50 til 100 μg GnRH.

En af forsøgskvierne svarede ikke på GnRH behandlingerne. Mu-lighed for forekomst af en individuel GnRH taerskeldosis er omtalt og fører til den hypotese, at stigende GnRH dosis snarere medfører, at en større andel af dyrene svarer på en behandling, end at det enkelte dyr giver større respons.

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Authors and Affiliations

  1. Institute of Animal Reproduction and Department of Forensic and State Veterinary Medicine, Royal Veterinary and Agricultural University, Copenhagen, Denmark

    A. Mikél Jensen, K. Myrup Pedersen, J. F. Agger & A. Madej

  2. Department of Clinical Chemistry, College of Veterinary Medicine, Swedish University of Agricultural Science, Uppsala, Sweden

    A. Mikél Jensen, K. Myrup Pedersen, J. F. Agger & A. Madej

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  1. A. Mikél Jensen
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  4. A. Madej
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Mikél Jensen, A., Pedersen, K.M., Agger, J.F. et al. Plasma Luteinizing Hormone Response to Increasing doses of Synthetic Gonadotrophin-Releasing Hormone in Heifers. Acta Vet Scand 24, 211–224 (1983). https://doi.org/10.1186/BF03546748

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Acta Veterinaria Scandinavica

ISSN: 1751-0147