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Wild Animal Mycobacterial Isolates

Karakterisering av mykobakterier isolert fra ville dyr ved analyse au fettsyrer og polare lipider

Characterization by Cellular Fatty Acid Composition and Polar Lipid Patterns

Acta Veterinaria Scandinavica volume 24, pages 225–237 (1983)Cite this article

Abstract

Thirteen strains of mycobacteria isolated from deer and various species of wild birds were analysed by gas chromatography (GG) for cellular fatty acids and by thin-layer chromatography (TLG) for polar lipids. These strains were compared to reference strains of Mycobacterium avium, M. para tuberculosis and M. mal-moense. All the examined strains exhibited a generally similar fatty acid pattern characterized by relatively large amounts of hexadenca-noate (16:0), octadecenoate (18:1), octadecanoate (18:0) and 10-me-thyl-octadecanoate (tuberculostearic acid, 10-Me-18:0). Several additional acids were also generally present but in smaller amounts. By means of small but distinct differences in fatty acid composition, the wild animal isolates could be distinguished from both M. paratuber-culosis and M. malmoense but not from M. avium.

The TLG polar lipid patterns on the other hand separated the wild animal isolates into 2 distinct groups of complex and simple polar lipid composition which corresponded to the morphologically smooth and rough types, respectively. The complex patterns of the smooth strains were comparable to those of the M. avium serovars whereas both the rough wild animal isolates and all the M. paratuber-culosis strains showed a simple pattern of polar lipids.

Both fatty acid profiles and TLG polar lipid patterns support allocation of the wild animal isolates to the MAIS complex. Moreover, the 2 chemical techniques, particularly the GC procedure, are very useful for a more rapid and precise identification of the slow-growing wild animal mycobacterial isolates which have hitherto been characterized on basis of vague criteria.

Sammendrag

Tretten ukjente stammer av mykobakterier isolert fra rådyr og ville fugler ble analysert med henblikk på fettsyrer og polare lipider og sammenliknet med referensestammer av Mycobacterium avium, M. paratuberculosis og M. malmoense. Alle undersøkte stammer hadde stort sett sammenliknbare fettsyremønstre karakterisert av relativt store kvanta av heksadekanoat (16:0), oktadekenoat (18:1), oktade-kanoat (18:0), og 10 methyl-oktadekanoat (tuberkulostearinsyre, 10-Me-18:0). Andre syrer ble også påvist, men i mindre kvanta. Ved hjelp av små, men distinkte forskjeller i fettsyresammensetning kunne de ukjente mykobakterieisolatene adskilles fra M. paratuberculosis og M. malmoense, men ikke fra M. avium.

Analyse av polare lipider (ved tynnsjiktkromatografi) separerte de ukjente stammer i 2 grupper av henholdsvis enkle og komplekse lipidmønstre som tilsvarte ru og glatt kolonimorfologi. Det komplekse mønsteret fra stammer med glatt kolonimorfologi var av samme type som mønsteret fra M. avium-referensestammene, mens stammene med ru kolonimorfologi og alle M. paratuberculosis-stammene hadde et enklere lipidmønster.

Både analysene av fettsyresammensetning og polare lipider indi-kerer at mykobakterieisolatene fra rådyr og ville fugler tilhører MAIS-komplekset (M. avium — M. intracellulare — M. scrofulaceum). For øvrig er de 2 kjemiske metodene, særlig fettsyreanalyse, et velegnet hjelpemiddel for en hurtig og mer eksakt identifisering av slike eks-tremt sentvoksende mykobakteriestammer.

References

  • Andersen, O., E. Jantzen, O. Closs, M. Harboe, F. Saxegaard & F. H. Fodstad: Fatty acid and polar lipid analysis as tools in the identification of Mycobacterium leprae and some related slow-growing mycobacterial species. Ann. Microbiol. (Inst. Pasteur) 1982, 133 B, 29–37.

    CAS  Google Scholar 

  • Brennan, P. J., M. Heifets & B. P. Ullom: Thin-layer chromatography of lipid antigens as a means of identifying non tuberculous mycobacteria. J. clin. Microbiol. 1982, 15, 447–455.

    Article  CAS  Google Scholar 

  • Brennan, P. J., M. Souhrada, B. Ullom, J. K. McClatchy & M. B. Goren: Identification of atypical mycobacteria by thin-layer chromatography of their surface antigens. J. clin. Microbiol. 1978, 8, 374–379.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Chomarat, M., J. P. Flandrois & J. Viallier: Analyse des acides gras des mycobactéries par chromatographic gaz-liquide. Étude de M. avium et de 20 autre espéces. (Analysis of fatty acids of mycobacteria by means of gas-liquid chromatography. A study of M. avium and 20 other species). Zbl. Bakt. Hyg., I. Abt. Orig. G 1981, 2, 21–32.

    CAS  Google Scholar 

  • Guerrant, G. O., M. S. Lambert & C. W. Moss: Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria. J. clin. Microbiol. 1981, 13, 890–907.

    Article  Google Scholar 

  • Jantzen, E., K. Bryn, N. Hagen, T. Bergan & K. Bøure: Fatty acid and monosaccharides of Neisseriaceae in relation to established taxonomy. NIPH Annals (Oslo) 1978, 1, 59–71.

    CAS  Google Scholar 

  • Jenkins, P. A.: Lipid analysis for the identification of mycobacteria: An appraisal. Rev. inf. Dis. 1981, 3, 862–866.

    Article  CAS  Google Scholar 

  • Lecheualier, M. P.: Lipids in bacterial taxonomy — a taxonomist’s viewpoint. Grit. Rev. Microbiol. 1977, 5, 109–210.

    Google Scholar 

  • Matthews, P. R. J. & A. Sargent: The isolation of mycobacteria from the brown hare (Lepus europaeus). Brit. vet. J. 1977, 133, 399–404.

    Article  CAS  Google Scholar 

  • Matthews, P. R. J., A. McDiarmid & P. Collins: Mycobacterial infections in various species of deer in the United Kingdom. Brit, vet. J. 1981, 137, 60–66.

    Article  CAS  Google Scholar 

  • McDiarmid, A.: Tuberculosis in wild birds. Proc. Roy. Soc. Med. 1964, 57, 480–481.

    Article  CAS  Google Scholar 

  • McDiarmid, A.: Some diseases of free-living wildlife. Adv. vet. Sci. 1975, 19, 97–126.

    CAS  Google Scholar 

  • Saxegaard, F.: Serological investigations of Mycobacterium avium and M. avium-like bacteria isolated from domestic and wild animals. Acta. vet. scand. 1981, 22, 153–161.

    CAS  PubMed  Google Scholar 

  • Schaefer, W. B.: Serologic identification and classification of the atypical mycobacteria by their agglutination. Amer. Rev. resp. Dis. 1965, 92, 85–93.

    CAS  PubMed  Google Scholar 

  • Sehrt, I., W. Käppler & A. Lange: Zur Differenzierung und Identifizie-rung von Mykobakterien mit Hilfe der Lipid-Dünnschichtchro-matographie. II. Anwendung bei langsam wachsenden Mykobakterien: M. avium und M. intracellulare. (Differentiation and identification of mycobacteria by means of thin-layer chromatography of their lipids. II. Application for the classification of slowly growing mycobacteria: M. avium and M. intracellulare). Z. Erkr. Atm. 1976, 144, 146–154.

    CAS  Google Scholar 

  • Thoen, C. O., A. G. Karlson & R. D. Ellefson: Comparison of gas-liquid chromatography of the fatty acids of Mycobacterium avium and some other nonphotochromogenic mycobacteria. Appl. Microbiol. 1971, 22, 560–563.

    Article  CAS  Google Scholar 

  • Thorel, M. F. & T. Desmettre: Étude comparative de souches de myco-bactéries mycobactine dépendentes, isolées de pigeon ramier, avec Mycobacterium avium et M. paratuberculosis : Étude de caractéres biologiques et anitigéniques. (Comparative study of mycobactin dependent strains of mycobacteria isolated from wood-pigeon with Mycobacterium avium and M. paratuberculosis). Ann. Microbiol. (Inst. Pasteur) 1982, 133 B, 291–302.

  • Tisdall, P. A., D. R. De Young, G. D. Roberts & J. P. Anhalt: Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone: a 10-month follow-up study. J. clin. Microbiol. 1982, 16, 400–402.

    Article  CAS  Google Scholar 

  • Tisdall, P. A., G. D. Roberts & J. P. Anhalt: Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone. J. clin. Microbiol. 1979, 10, 506–514.

    Article  CAS  Google Scholar 

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  1. National Veterinary Institute and National Institute of Public Health, P. O. Box 8156, Dep., Oslo 1, Norway

    Finn Saxegaard, Otto Andersen & Erik Jantzen

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  1. Finn Saxegaard
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  2. Otto Andersen
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  3. Erik Jantzen
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Saxegaard, F., Andersen, O. & Jantzen, E. Wild Animal Mycobacterial Isolates. Acta Vet Scand 24, 225–237 (1983). https://doi.org/10.1186/BF03546749

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Acta Veterinaria Scandinavica

ISSN: 1751-0147