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Electrophoretic Analysis of Isoenzymes of Mycoplasma Species

Elektroforetisk analyse af isoenzymer hos mykoplasmer

Acta Veterinaria Scandinavica volume 24, pages 14–33 (1983)Cite this article

Abstract

The purpose of this paper is to further characterize and determine relatedness among some Mycoplasma species or serogroups of significant practical veterinary interest. Twenty-four strains were examined for the presence of 35 enzymes by horizontal starch gel electrophoresis, revealing a total of 127 different electromorphs of 30 enzymes. Inter- as well as intraspecific differences were found demonstrating the application of isoenzyme studies in classification as well as epidemiology. It is concluded that the F38 group of MacOwan and group 7 of Leach constitute 2 new species, The elevation of the 2 subspecies of M. mycoides (mycoides and capri) to species level is favoured, but it is suggested that a decision be taken internationally, considering the practical consequences of a given nomenclature. Three alternative possibilities for classification are presented.

Regarding identification the results suggest that the presence or absence of maltase and ornithine transcarbamylase indicate whether an isolate is related to the agent of contagious bovine pleuropneumonia or merely a representative of either the caprine LG type of M. mycoides subsp. mycoides or the classical caprine subspecies, M. mycoides subsp. capri.

Sammendrag

Formålet med dette arbejde er, ved hjælp af isoenzymstudier, yder-ligere at karakterisere og vurdere slægtsskabet af visse grupper eller arter af mykoplasmer, som har betydelig veterinser interesse i sygdoms-msessig henseende. Ved horisontal stivelseselektroforese blev 24 stammer undersøgt for tilstedevserelse af 35 enzymer. Der påvistes 127 elektromorfer blandt 30 enzymer. Inter- såvel som intraspecifikke for-skelle demonstrerede anvendelsesmuligheder for isoenzymsitudier i henseende til både klassifikation og epidemiologi.

Det konkluderes, at den caprine gruppe F38 (MacOwan) og bovin gruppe 7 (Leach) begge bør opfaites som nye, selvstændige arter. I henseende til den taksonomiske status af de 2 underarter af M. my-coides — subspecies mycoides og subspecies capri — vil forfatterne anbeifale, at de klassificeres som selvstaendige arter. Det forestås dog, at beslutningen tages på internationalt niveau på grund af de praktiske konsekvenser af en given nomenklatur, netop ved denne gruppe af mykoplasmer. Der vises 3 forskellige muligheder for alternativ nomenklatur.

Med hensyn til rutinemæssig identifikation synes resultaterne at åbne mulighed for, at undersøgelse for maltase og ornithintranscarba-mylase kan indicere, hvor vidt en given stamme er at betragte som tilhørende den gruppe, som er årsag til oksens ondartede lungesyge.

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  1. Institute of Medical Microbiology and Institute of Ecology and Genetics, University of Aarhus, Bartholin Building, DK-8000, Aar-hus C, Denmark

    M. M. Salih, H. Ernø & V. Simonsen

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  1. M. M. Salih
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  2. H. Ernø
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  3. V. Simonsen
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Salih, M.M., Ernø, H. & Simonsen, V. Electrophoretic Analysis of Isoenzymes of Mycoplasma Species. Acta Vet Scand 24, 14–33 (1983). https://doi.org/10.1186/BF03546754

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Acta Veterinaria Scandinavica

ISSN: 1751-0147