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Comparison Between Elisa and Dye Test for Detection of Naturally Acquired Toxoplasma Gondii Antibodies in the Goat

Sammenligning mellom ELISA og vitalfarging for påvisning av anti-sloffer mot Toxoplasma gondii hos naturlig infiserte geit

Acta Veterinaria Scandinavica volume 24, pages 65–73 (1983)Cite this article

Abstract

Toxoplasma gondii IgG antibodies were measured in 212 goat sera, comparing the Sabin-Feldman dye-test and a three-layer sandwich enzyme-linked immunosorbent assay (ELISA). With 98 % concordance obtained between these 2 tests, the results are at the same paragon as for human sera. Accordingly, the ELISA sandwich procedure appears to be suitable for large-scale analysis of goat sera. The discordant 2 % were ELISA positive and dye-test negative. One possible explanation of the divergent titres is given using an immunized goat model.

Sammendrag

IgG antistoffer mot Toxoplasma gondii ble malt i 212 geit ved hjelp av Sabin-Feldman’s dye test, og en tre-lags sandwich ELISA (“enzyme-linked immunosorbent assay”). Med 98 % sammenfallende resultater i disse 2 testene, var overensstemmelsen på lik lin je med hva man finner for humane sera. Derav følger at ELISA prosedyren

også bør egne seg for masseundersøkelser av geitesera.

De 2 % avvik var ELISA positive og dye-test negative. En mulig forklaring på denne divergensen er be&krevet ved hjelp av en immuni-sert geit-modell.

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Authors and Affiliations

  1. Norwegian Defence Microbiological Laboratory, National Inisitute of Public Healh, Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, and the Department of Animal Breeding, Agricultural University of Norway, Geitmyrsveien 75, Oslo 4, Norway

    Bjørn P. Berdal, Ørjan Olsvik, Torbjørn Alrnlid, Hans Jørgen Larsen & Anne-Marie Lorentzen-Styr

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  1. Bjørn P. Berdal
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  2. Ørjan Olsvik
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  3. Torbjørn Alrnlid
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  4. Hans Jørgen Larsen
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  5. Anne-Marie Lorentzen-Styr
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Berdal, B.P., Olsvik, Ø., Alrnlid, T. et al. Comparison Between Elisa and Dye Test for Detection of Naturally Acquired Toxoplasma Gondii Antibodies in the Goat. Acta Vet Scand 24, 65–73 (1983). https://doi.org/10.1186/BF03546758

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Acta Veterinaria Scandinavica

ISSN: 1751-0147