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An Improved Medium for Culture of Mycoracterium Paratuberculosis from Bovine Faeces

Et forbedret substrat til dyrkning af Mycobacterium paratuberculosis fra kvseggødning

Acta Veterinaria Scandinavica volume 23, pages 325–335 (1982)Cite this article

Abstract

Löwenstein-Jensen medium with mycobactin, cyclo-heximide, penicillin, and chloramphenicol, and enriched with sodium pyruvate, was compared with an ordinary L.-J. medium with mycobactin. Faeces samples from cattle experimentally infected with M. paratuberculosis were cultured on both media. The improved medium gave 11 % more positive cultures and 90 % more colonies. Of the positive cultures 97 % showed detectable growth after 8 weeks of incubation, and the contamination rate was reduced to 0.4 %. By culture of faeces samples from naturally infected cattle the improved medium identified 23.2 % more infected animals than the basic medium, mostly due to a reduction of the contamination rate to about 3 %.

Sammendrag

Löwenstein-Jensen substrat med mykobaktin, cycloheximide, penicillin, chloramphenicol og pyrodruesyre blev sammenlignet med et ordinaert L.-J. substrat med mykobaktin. Gødningsprøver fra kalve oralt inficeret med M. paratuberculosis blev dyrket på begge substra-ter. På det forbedrede substrat fandtes 11 % flere positive kulturer. På 97 % af de positive kulturer fandtes tydelig kolonidannelse efter 8 ugers inkubation, og procenten af forurenede kulturer blev redu-ceret til 0,4. Ved dyrkning af gødningsprøver fra naturligt inficerede kreaturer blev diagnosticeret 23,2 % flere inficerede dyr på det forbedrede end på det ordinære substrat. Det bedre resultat skyldes hovedsageligt en reduktion af forurenede kulturer til ca. 3

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  1. State Veterinary Serum Laboratory, Rülowsvej 27, Copenhagen, DK-1870, Denmark

    J. Berg Jørgensen

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  1. J. Berg Jørgensen
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This work was supported by a grant from the Danish Agricultural and Veterinary Research Council.

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Jørgensen, J.B. An Improved Medium for Culture of Mycoracterium Paratuberculosis from Bovine Faeces. Acta Vet Scand 23, 325–335 (1982). https://doi.org/10.1186/BF03546784

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Acta Veterinaria Scandinavica

ISSN: 1751-0147