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Comparison of Different Agar Diffusion Methods for the Detection of Antimicrobial Residues in Slaughter Animals
Jämförelse mellan olika agar-diffusions metoder för påvisning av antimikrobiella rester i slaktkroppar
Acta Veterinaria Scandinavica volume 23, pages 407–415 (1982)
Abstract
The different agar diffusion methods were compared using antibiotic and sulphonamide-impregnated filter-paper discs and the kidneys of healthy and emergency-slaughtered pigs and cows after slaughter.
No method used seemed to be sensitive to all antimicrobial drugs preimpregnated onto discs. Tetracycline yielded a greater zone of inhibition at pH 6 than at pH 8 and aminoglycosides, erythromycin, polymyxin B and lin cornycin at pH 8 than at pH 6. It therefore seems necessary to use different pHs (6 and 8). The addition of trimethoprim to the medium is necessary for the detection of sulphonamides. Bacillus subtilis BGA used as the test organism was more sensitive to sulphonamides on the “Test agar for the inhibitor test” containing trimethoprim than on the “Iso-Sensitest agar” also containing trimethoprim. The addition of trimethoprim to “Test agar for the inhibitor test” is recommended at pH 8 but not at pH 6 because false-positive cases (with inhibition zones > 2 mm) were observed at pH 6 with trimethoprim on the kidneys of healthy pigs.
Sammanfattning
Olika agar-dif fusions metoder jämfördes genom att an vända filter-papperdisker innehållande antimikrobiella läkemedel, och njurar från friska och nödslaktade slaktsvin.
Ingen använd metod syn-tes vara känslig for alia antimikrobiella läkemedel, som hade impregnerats på diskerna. Tetracyklin förorsa-kade en större hämningszon vid pH 6 an vid pH 8 och aminoglyco-sider, erytromycin, polymyxin B och lincomycin vid pH 8 an vid pH 6. Det synes därför nödvändigt att använda två olika pH-värden (6 och 8). Genom tillsats av trimetoprim var det möjligt att bestämma sulfonamider. Bacillus subtilis BGA var känsligare för sulfonamider på „Test agar for the inhibitor test”, som innehöll trimetoprim, an på „Iso-Sensitest agar”, som också innehöll trimetoprim. Tillsats av trimetoprim rekommenderas vid pH 8 men icke vid pH 6. Falska-positiva fall (hämningszoner > 2 mm) observerades vid pH 6 med trimetoprim, när njurar av svin underssöktes.
References
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Korkeala, H., Sorvettula, O., Mäki-Petäys, O. et al. Comparison of Different Agar Diffusion Methods for the Detection of Antimicrobial Residues in Slaughter Animals. Acta Vet Scand 23, 407–415 (1982). https://doi.org/10.1186/BF03546792
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DOI: https://doi.org/10.1186/BF03546792