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Effect of Recovery Medium on the Isolation of Campylobacter Jejuni Before and After Heat Treatment

Effekten av växtmedium på Isolationen av Campylobacter jejuni före och efter uärmebehandling

Acta Veterinaria Scandinavica volume 23, pages 416–424 (1982)Cite this article

Abstract

The effect of various concentrations of polymyxin B and Colistin in Skirrow’s or Butzler’s type media, respectively, on the recovery rate of 13 strains of G. jejuni before and after heat treatment was studied. Prior to heating, a Butz-ler’s type medium containing 40 I.U. per ml Colistin was inhibitory for certain strains of C. jejuni. After heating at 48° G for 30 min, media containing 2.5 I.U. per ml polymyxin B or 10 I.U. per ml Colistin were not inhibitory for heat-injured cells. When the concentrations of polymyxin B were increased to 5.0 I.U. per ml or those of Colistin to 20 or 40 I.U. per ml in the selective media, the means of the differences of log cell counts between non-selective Brucella blood agar and the selective media was statistically significant (P < 0.05). The mean D-value of G. jejuni strains in Brucella broth at 48° G was 18.4±5.4 min.

Sammanfattning

Effekten av olika koncentrationerna av polymyxin B och Colistin i Skirrows eller Butzlers media på aterhämtningsgraden hos 13 stam-mar av Campylobacter jejuni före och efter värmebehandling under-söktes. Före upphettning hade Butzlers medium innehållande 40 I.U. colistin/ml en inhiberande effekt på vissa G. jejuni stammar. Efter upphettning vid 48°C under 30 min. var media innehållande 2.5 I.U./ ml polymyxin B eller 10 I.U./ml Colistin inte inhiberande för värme-skadade celler. När polymyxin B koncentrationen höjdes till 5.0 I.U./ ml och Colistin koncentrationen till 20 eller 40 I.U./ml i selektiva media, var medeltalen av skillnaderna i log cell uträkningarna mellan nonselektiva Brucella blodagar och de selektiva medierna statistiskt signifikanta (P < 0,05). Medeltalet av D-värdet för C. jejuni stammar vid 48 °G var 18.4 ± 5.4 min.

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  1. Department of Food Hygiene, College of Veterinary Medicine, Hämeentie 57, Helsinki, 00550, Finland

    Marja-Liisa Hänninen

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Hänninen, ML. Effect of Recovery Medium on the Isolation of Campylobacter Jejuni Before and After Heat Treatment. Acta Vet Scand 23, 416–424 (1982). https://doi.org/10.1186/BF03546793

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Acta Veterinaria Scandinavica

ISSN: 1751-0147