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Comparison of Four Enrichment Media in the Recovery of Campyloracter Jejuni

En jämförelse mellan fyra anrikningsmedier för påuisnina av Campylobacter jejuni

Acta Veterinaria Scandinavica volume 23, pages 425–437 (1982)Cite this article

Abstract

The effectiveness of 4 enrichment media for the recovery of low levels of inoculated cells of Campylobacter jejuni was evaluated. The media contained antibiotics or antibiotics and bile acids as selective compounds. Three of the media recovered most of the inoculated low numbers of 6 C. jejuni strains. In the 3 media the growth rate of 3 strains, indicated by the increase in the log number of cells during 24 h or 48 h incubation at 42 ° C, was about the same as in the control medium without selective compounds. The same 3 media also recovered a low number of Campylobacter cells from artificially contaminated raw milk or ground meat samples. The enrichment medium B containing 40 I.U. Colistin, 5 μg novobiocin, 2 mg Na-cholic acid and 50 mg cycloheximide per ml was inhibitory for most Campylobacter strains studied.

Sammanfattning

Fyra olika anrikningsmedier för påvisning av små mängder av C. jejuni bar jämförts. Medierna innehöll antimikrobiella ämnen eller antimikrobiella ämnen och gallsyror som selektiva faktorer. I tre av dessa medier var bade växten av C. jejuni stammar i renkulturer och påvisningen av C. jejuni från artificiellt kontaminerat malet kött eller rå mjölk bra. I det fjärde anrikningsmediet, som innehöll 40 I.U. koli-stin, 5 μg novobiocin, 2 mg Na-cholin syra och 50 mg cyclohexamid per ml, inhiberades tillväxten av de fiesta undersökta C. jejuni stam-marna.

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  1. Department of Food Hygiene, College of Veterinary Medicine, Hämeentie 57, Helsinki, 00550, Finland

    Marja-Liisa Hänninen

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  1. Marja-Liisa Hänninen
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Hänninen, ML. Comparison of Four Enrichment Media in the Recovery of Campyloracter Jejuni. Acta Vet Scand 23, 425–437 (1982). https://doi.org/10.1186/BF03546794

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Acta Veterinaria Scandinavica

ISSN: 1751-0147