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Detection of Staphylococcal Enterotoxin A, B, and C in Milk By an Elisa Procedure

Påvisning av staphylococcal enterotoxin A, B og C i melk ved en ELISA metode

Acta Veterinaria Scandinavica volume 23, pages 204–210 (1982)Cite this article

Abstract

Enterotoxigenic reference strains of Staphylococcus aureus were cultivated in sterile whole and skim milk for 18 h at 37°G. Staphylococcal enterotoxin A, B, and C were detected directly in the milk by an enzyme linked immunosorbent assay (ELISA), sensitive down to 1 ng/ml. Enterotoxins in the range of 1 ng–20 µg/ml milk were detected without any concentration or extraction. Skim and whole milk were almost identical as medium for enterotoxin production.

Sammendrag

Stafylokokk enterotoxin A, B og C (SEA, SEB, SEC) ble målt i melk direkte ved en firelags enzym-merket immunologisk metode (ELISA). Ved å tilsette renset SEA, SEB eller SEC til skummet og hel melk, var det mulig å bestemme toxinmengder ned til 1 ng/ml. Enterotoksiske Staphylococcus aureus ble inokulert i steril hel og skummet melk og dyrket ved 37° G i 18 timer. Enterotoxin (A, B og C) mengden ble besternt til 1 ng–20µg per ml, og det var liten forskjell på enterotoxin produksjonen i hel og skummet melk.

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Authors and Affiliations

  1. Norwegian Defence Microbiological Laboratory, National Institute of Public Health, Department of Microbiology and Immunology, Veterinary College of Norway, Oslo, Norway

    Ørjan Olsvik, Stein Myhre, Bjorn P. Berdal & Kåre Fossum

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  1. Ørjan Olsvik
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  2. Stein Myhre
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  3. Bjorn P. Berdal
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  4. Kåre Fossum
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Olsvik, Ø., Myhre, S., Berdal, B.P. et al. Detection of Staphylococcal Enterotoxin A, B, and C in Milk By an Elisa Procedure. Acta Vet Scand 23, 204–210 (1982). https://doi.org/10.1186/BF03546806

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Acta Veterinaria Scandinavica

ISSN: 1751-0147