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Serological, Enterotoxin-Producing and Biochemical Properties of Escherichia Coli Isolated from Piglets with Neonatal Diarrhea in Norway

Serologiske, enterotoksin-produserende og biokjemiske egenskaper hos Escherichia coli isolert fra spedgriser med diare i Norge

Acta Veterinaria Scandinavica volume 23, pages 79–87 (1982)Cite this article

Abstract

Altogether 235 strains of Escherichia coli isolated from jejunal content of piglets with neonatal diarrhea were examined for serological, enterotoxin-producing and certain biochemical properties. Of 198 strains examined, 84 (42 %) belonged to O-group 149, while 14 (7 %) strains belonged to each of the O-group s 8 and 64. Seventy strains (30%) could not be grouped with the sera used. The remaining strains were distributed among the following O-groups with only a few strains in each group: 2,6,9,32,45,98 and 141. Eighty out of 84 E. coli strains of O-group 149 possessed the K88 antigen and produced heat labile enterotoxin (LT). Besides, LT production was demonstrated in 3 out of 14 strains of E. coli 08. K88 antigen was demonstrated in only 1 strain not belonging to O-group 149. Among strains of E. coli 064 12 out of 14 were K99 positive. This antigen was not demonstrated in E. coli strains of other O-groups. A close relationship was demonstrated between strains of E. coli 0149 possessing the K88 antigen and the ability to ferment both raffinose and adonitol. This ability was only detected in 2 other strains of E. coli not belonging to O-group 149.

Sammendrag

Tilsammen 235 stammer av Escherichia coli isolert fra tynntarminnhold fra spedgriser med diare ble undersøkt for serologiske og visse biokjemiske egenskaper, samt for evne til å produsere termolabilt enteroloksin (LT). Blant 19 stammer tilhørte 84 (42 %) O-gruppe 149, mens 15 (7 %) stammer tilhørte O8, og det samme antall tilhørte O64. Det var ikke mulig å plassere 70 (ca. 35 %) av stammene i O-gruppe med de benyttede antiseraer. Få stammer ble funnet å tilhøre følgende ø-grupper: 2,6,9,32,45,98 og 141. Åtti av 84 E. coli stammer tilhørende O-gruppe 149 hadde K88 antigen og produserte LT. Blant stammene tilhørende O64 var 12 av 14 positive med hensyn til K99 antigenet. Dette adhesinet ble ikke påvist hos andre ø-grupper av E. coli, mens K88 antigenet ble påvist hos 1 stamme tilhørende en ukjent ø-gruppe foruten hos E. coli O149. Produksjon av LT ble i tillegg påvist hos 3 av 14 stammer tilhørende O-gruppe 8.

Av 101 stammer tilhørende O-gruppe 149 hadde 100 evne til å forgjære raffinose og adonitol samtidig. Nittiåtte av disse var K88 positive. Evnen til å omsette raffinose og adonitol samtidig ble forøvrig bare påvist hos 2 andre stammer tilhørende ukjente O-grupper.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Veterinary College of Norway, P. O. Box 8146, Dep., Oslo 1, Oslo, Norway

    Arve Lund, Kåre Fossum & Eivind Liven

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  1. Arve Lund
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  2. Kåre Fossum
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  3. Eivind Liven
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This investigation was made possible by financial support from the Agricultural Research Council of Norway.

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Lund, A., Fossum, K. & Liven, E. Serological, Enterotoxin-Producing and Biochemical Properties of Escherichia Coli Isolated from Piglets with Neonatal Diarrhea in Norway. Acta Vet Scand 23, 79–87 (1982). https://doi.org/10.1186/BF03546824

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Acta Veterinaria Scandinavica

ISSN: 1751-0147