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Cryopreservation of Hen Red Blood Cells
Beskyttelse af røde høneblodlegemer ved frysing
Acta Veterinaria Scandinavica volume 21, pages 498–503 (1980)
Abstract
The cryoprotection of hen erythrocytes, used as reagent in virus titration, was investigated. The cryoprotective agents tested were neutralized polyvinylpyrrolidone (PVP), dimethylsulfoxide (DMSO) and glycerol. Good results were obtained with PVP, especially with PVP K15 (average molecular weight 10 000), and with DMSO, especially when used in a final concentration of 10 %, whereas glycerol was unfit for use in the concentrations tested. The red cell concentration, the suspending buffer before freezing and the washing procedure after thawing were of importance. The cells could be frozen and stored for at least three months without any significant effect on the virus titer.
Sammendrag
Bevaring av røde høneblodlegemer mot skade etter nedfrysing til –196°C ble undersøkt. Slike celler brakes som reagens for titrering av virus. Som kryoprotektive midier ble brukt nøytralisert polyvinylpyrrolidone (PVP), dimetylsulfoxide (DMSO) og glycerol. God celle-gjenvinning ble oppndd med PVP, isaer med PVP Κ 15 (molekylvekt 10 000), og med DMSO når denne ble brukt i endelig konsentrasjon 10 %, mens glycerol var uegnet. Cellekonsentrasjonen, suspensjons-middelet før frysing og vaskeprosedyren etter tining var av betydning. Virustitreringer med frosne celler ga samme resultat som nr ufrosne celler ble brukt som reagens.
References
Myhrvold, V.: Cryopreservation of sheep red blood cells. 1. Experiments with polyvinylpyrrolidone and other protective agents. Acta vet. scand. 1979a, 20, 525–530.
Myhrvold, V.: Cryopreservation of sheep red blood cells. 2. Purified polyvinylpyrrolidone and hydrolyzed starch as protective agents. Acta vet. scand. 1979b, 20, 531–536.
Myhrvold, V.: Gryopreservation of sheep red blood cells. 3. Complement titrations with frozen sensitized cells. Acta vet. scand. 1979c, 20, 537–545.
Myhrvold, V.: Cryopreservation of sheep red blood cells. 4. Titrations of the first complement component with frozen EAC4 cells. Acta vet. scand. 1980, 21, 291–298.
Wesslén, L.: Hemolysis-in-gel (HIG) test for antibodies to influenza A, measles and mumps using liquid nitrogen freezed erythrocytes coupled with the respective viral antigen. J. Immunol. Methods 1978, 24, 1–8.
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Myhrvold, V. Cryopreservation of Hen Red Blood Cells. Acta Vet Scand 21, 498–503 (1980). https://doi.org/10.1186/BF03546837
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DOI: https://doi.org/10.1186/BF03546837