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The Use of a “Tt” Shaped Cannula for Collection of Duodenal Digesta in Sheep
Bruk αν „TT”-formet kanyle for oppsamling αυ chymus fra tolvfingertarmen hos sau
Acta Veterinaria Scandinavica volume 21, pages 546–558 (1980)
Abstract
Six sheep were equipped with both a rumen fistula and a duodenal cannula. The duodenal cannula was made of two ordinary T shape cannulas which were vulcanized into a double T cannula. Both the rumen and the duodenal cannulas were made of soft rubber.
In two experiments six different diets were fed. In the first experiment the diets consisted mainly of ammonia-treated straw (NH3-straw) plus barley or sodium hydroxide-treated straw (NaOH-straw) plus concentrate containing different N-supplements. In the second experiment, the treated straw (either NH3-straw or NaOH-straw) was fed alone. The animals in the two experiments were fed at maintenance level.
Duodenal digesta were collected for periods of 12 h by inflating a balloon in the distal flange of the cannula, using pieces of foam rubber. The volume of fluid leaving the rumen was measured using the marker Gr-EDTA. The effect of digesta removal on blood constituents was studied. Three to four observations on each ration were made.
Digesta flow and dry matter entering the duodenum were higher with NH3-straw than NaOH-straw fed either alone or with concentrate. However, there was a considerable variation of which a large part was of individual nature.
The volume of fluid reaching the duodenum was always lower than the volume leaving the rumen, indicating a net absorption of water in the omasum and abomasum. This was estimated to be from 10.1 to 11.6 1/24 h in Experiment 1 and from 0.3 to 1.4 1/24 h in Experiment 2 (51–59 and 5–31 % of the ruminai outflow, respectively).
A significant increase in plasma K and Mg and decrease in Pi and a-amino N concentration were observed due to 12 h collection of duodenal digesta. However, plasma Na, Ca, glucose and urea nitrogen concentrations remained at their pre-collection levels.
Post-mortem examination of duodenum in sheep slaughtered after six and 10 months revealed a normal gut with no sign of dilatation in the immediate vicinity of the cannula.
Sammendrag
Seks sauer ble utstyrt med både vomfistel og en kanyle i tolvfingertarmen. Tolvfingertarm-kanylen besto av to vanlige T-formede kanyler som var vulkanisert sammen til en dobbel T-kanyle. Både vomfistlene og tolvfingertarm-kanylene var laget av mjuk gummi.
Forsøksdyrene ble gitt seks forskjellige fórrasjoner i to forsøk. Rasjonene i det første forsøket besto hovedsaklig av ammoniakkbehandla halm (NH3-halm) plus byggrøpp eller natriumhydroksyd-behandla halm (NaOH-halm) plus kraftfór med forskjellig N-tilskudd. I det andre forsøket ble behandlet halm (enten NH3-halm eller NaOH-halm) gitt som eneste fór. Forsøksdyrene ble fóret til vedlikeholdsnivå.
Chymus fra tolvfingertarmen ble samlet opp i 12 timer ved å føre en ballong gjennom den distale åpningen av kanylen, fylle denne med skumgummi og derved blokkere passasjen videre bakover gjennom tarmen. Mengden av vaeske som forlot vom/nettmage ble målt ved å bruke Cr-EDTA som markør. Endringen i en del blodkomponenter ved fjerning av chymus ble også undersøkt og det ble utført tre til fire malinger for hver fórrasjon.
Mengden av total chymus og tørrstoff som strømmet inn i tolvfingertarmen var større for NH3-halm enn for NaOH-halm både med og uten kraftfórtilskudd. Det var imidlertid en betydelig individuell variasjon i materialet.
Mengden av vaeske som ndde tolvfingertarmen var alltid lågere enn mengden som passerte ut av vom/nettmage. Dette indikerer en netto absorpsjon av vann i bladmage og løpe, og den ble beregnet til 10.1–11.6 1/24 t i forsøk 1 og 0.3–1.4 1/24 t i forsøk 2 (henholdsvis 51–59 og 5–31 % av mengden som forlot vom/nettmage).
Det ble funnet en signifikant økning i konsentrasjonen av plasma Κ og Mg, og nedgang i Pj og α-amino Ν ved fjerning av chymus fra tolvfingertarmen i 12 t. Konsentrasjonen av plasma Na, Ca, glukose og urea avvek ikke fra nivet før fjerningen av chymus.
Post-mortem undersøkelser av tolvfingertarmene hos sauer slaktet 6 og 1ø mneder etter fistulering viste at tarmen var normal uten tegn til dilatasjon rundt kanylen eller i naermeste tarmavsnitt.
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Borhami, B.E.A., Hove, K. & Sundstøl, F. The Use of a “Tt” Shaped Cannula for Collection of Duodenal Digesta in Sheep. Acta Vet Scand 21, 546–558 (1980). https://doi.org/10.1186/BF03546842
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DOI: https://doi.org/10.1186/BF03546842