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Indirect Immunofluorescence Using F(ab’)2-Immuno-Reagents for the Demonstration of Bovine Viral Diarrhea Virus (Bvdv) Antigen in Lymphoid Tissue


  1. Biberfeld, P., G. Biberfeld, Z. Molnar & A. Fagraeus: Fixation of cell-bound antibody in the membrane immunofluorescence test. J. Immun. Meth. 1974, 4, 135–148.

  2. Eskildsen, M.: Complement fixation with porcine serum antibodies. Acta path, microbiol. scand. Sect. C 1975, 83, 315–324.

    Google Scholar 

  3. Grewal, A. S., B. T. Rouse & L. A. Babiuk: Characterization of surface receptors on bovine leukocytes. Int. Arch. Allergy 1978, 56, 289–300.

    CAS  Article  Google Scholar 

  4. Harboe, N. & A. Ingild: Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand. J. Immunol. 1973, 2, Suppl. 1, 161–104.

  5. Hijmans, W., H. R. E. Schuit & F. Klein: An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin, exp. Immunol. 1969, 4, 457–472.

  6. Kabat, E. A. & M. M. Mayer: Experimental Immunochemistry. 2nd Ed., Thomas, Springfield, Illinois 1964, 559–563.

  7. Kasukawa, R., M. Okada & S. Igari: Receptors for IgG Fc and complement in the mammalian aorta. Int. Arch. Allergy 1980, 61, 175–182.

    CAS  Article  Google Scholar 

  8. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 1970, 227, 680–685.

    CAS  Article  Google Scholar 

  9. Møller, N. P. H.: Fc-medicated immune precipitation. I. A new role of the Fc-portion of IgG. Immunology 1979, 38, 631–640.

    PubMed  Google Scholar 

  10. Ohmann, H. B., M. Holm Jensen, K. J. Sørensen & K. Dalsgaard: Demonstration of bovine viral diarrhea antigen in tissue sections by immunoperoxidase assay. In preparation.

  11. Poulsen, F. & T. Hjorth: A simple method for the production of F (ab’)2 preparations by pepsin digestion of total serum protein. Acta path, microbiol. scand. Sect. C 1980, 88, 241–245.

  12. Rossi, C. R. & G. K. Kiesel: Bovine immunoglobulin G subclass receptor sites on bovine macrophages. Amer. J. vet. Res. 1977, 38, 1023–1025.

    CAS  PubMed  Google Scholar 

  13. Rothlein, R., D. W. Johnson & C. C. Muscoplat: Enumeration and characterization of bovine blood, spleen and lymph node cells containing immunoglobulins. Int. Arch. Allergy 1980, 61, 100–106.

    CAS  Article  Google Scholar 

  14. The, T. H. & T. E. W. Feltkamp: Conjugation of fluorescein isothio-cyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 1970, 18, 865–873.

    CAS  PubMed  Google Scholar 

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Correspondence to Helle Bielefeldt Ohmann or Dr. Kristian Dalsgaard.

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Ohmann, H.B., Dalsgaard, K. Indirect Immunofluorescence Using F(ab’)2-Immuno-Reagents for the Demonstration of Bovine Viral Diarrhea Virus (Bvdv) Antigen in Lymphoid Tissue. Acta Vet Scand 21, 705–707 (1980).

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