Skip to main content

Indirect Immunofluorescence Using F(ab’)2-Immuno-Reagents for the Demonstration of Bovine Viral Diarrhea Virus (Bvdv) Antigen in Lymphoid Tissue

References

  1. Biberfeld, P., G. Biberfeld, Z. Molnar & A. Fagraeus: Fixation of cell-bound antibody in the membrane immunofluorescence test. J. Immun. Meth. 1974, 4, 135–148.

  2. Eskildsen, M.: Complement fixation with porcine serum antibodies. Acta path, microbiol. scand. Sect. C 1975, 83, 315–324.

    Google Scholar 

  3. Grewal, A. S., B. T. Rouse & L. A. Babiuk: Characterization of surface receptors on bovine leukocytes. Int. Arch. Allergy 1978, 56, 289–300.

    CAS  Article  Google Scholar 

  4. Harboe, N. & A. Ingild: Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand. J. Immunol. 1973, 2, Suppl. 1, 161–104.

  5. Hijmans, W., H. R. E. Schuit & F. Klein: An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin, exp. Immunol. 1969, 4, 457–472.

  6. Kabat, E. A. & M. M. Mayer: Experimental Immunochemistry. 2nd Ed., Thomas, Springfield, Illinois 1964, 559–563.

  7. Kasukawa, R., M. Okada & S. Igari: Receptors for IgG Fc and complement in the mammalian aorta. Int. Arch. Allergy 1980, 61, 175–182.

    CAS  Article  Google Scholar 

  8. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 1970, 227, 680–685.

    CAS  Article  Google Scholar 

  9. Møller, N. P. H.: Fc-medicated immune precipitation. I. A new role of the Fc-portion of IgG. Immunology 1979, 38, 631–640.

    PubMed  Google Scholar 

  10. Ohmann, H. B., M. Holm Jensen, K. J. Sørensen & K. Dalsgaard: Demonstration of bovine viral diarrhea antigen in tissue sections by immunoperoxidase assay. In preparation.

  11. Poulsen, F. & T. Hjorth: A simple method for the production of F (ab’)2 preparations by pepsin digestion of total serum protein. Acta path, microbiol. scand. Sect. C 1980, 88, 241–245.

  12. Rossi, C. R. & G. K. Kiesel: Bovine immunoglobulin G subclass receptor sites on bovine macrophages. Amer. J. vet. Res. 1977, 38, 1023–1025.

    CAS  PubMed  Google Scholar 

  13. Rothlein, R., D. W. Johnson & C. C. Muscoplat: Enumeration and characterization of bovine blood, spleen and lymph node cells containing immunoglobulins. Int. Arch. Allergy 1980, 61, 100–106.

    CAS  Article  Google Scholar 

  14. The, T. H. & T. E. W. Feltkamp: Conjugation of fluorescein isothio-cyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 1970, 18, 865–873.

    CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Helle Bielefeldt Ohmann or Dr. Kristian Dalsgaard.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ohmann, H.B., Dalsgaard, K. Indirect Immunofluorescence Using F(ab’)2-Immuno-Reagents for the Demonstration of Bovine Viral Diarrhea Virus (Bvdv) Antigen in Lymphoid Tissue. Acta Vet Scand 21, 705–707 (1980). https://doi.org/10.1186/BF03546858

Download citation