Skip to main content
Download PDF
  • Brief Communication
  • Published:

Indirect Immunofluorescence Using F(ab’)2-Immuno-Reagents for the Demonstration of Bovine Viral Diarrhea Virus (Bvdv) Antigen in Lymphoid Tissue

Acta Veterinaria Scandinavica volume 21, pages 705–707 (1980)Cite this article

References

  • Biberfeld, P., G. Biberfeld, Z. Molnar & A. Fagraeus: Fixation of cell-bound antibody in the membrane immunofluorescence test. J. Immun. Meth. 1974, 4, 135–148.

  • Eskildsen, M.: Complement fixation with porcine serum antibodies. Acta path, microbiol. scand. Sect. C 1975, 83, 315–324.

    Google Scholar 

  • Grewal, A. S., B. T. Rouse & L. A. Babiuk: Characterization of surface receptors on bovine leukocytes. Int. Arch. Allergy 1978, 56, 289–300.

    Article  CAS  Google Scholar 

  • Harboe, N. & A. Ingild: Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand. J. Immunol. 1973, 2, Suppl. 1, 161–104.

  • Hijmans, W., H. R. E. Schuit & F. Klein: An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin, exp. Immunol. 1969, 4, 457–472.

  • Kabat, E. A. & M. M. Mayer: Experimental Immunochemistry. 2nd Ed., Thomas, Springfield, Illinois 1964, 559–563.

  • Kasukawa, R., M. Okada & S. Igari: Receptors for IgG Fc and complement in the mammalian aorta. Int. Arch. Allergy 1980, 61, 175–182.

    Article  CAS  Google Scholar 

  • Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 1970, 227, 680–685.

    Article  CAS  Google Scholar 

  • Møller, N. P. H.: Fc-medicated immune precipitation. I. A new role of the Fc-portion of IgG. Immunology 1979, 38, 631–640.

    PubMed  Google Scholar 

  • Ohmann, H. B., M. Holm Jensen, K. J. Sørensen & K. Dalsgaard: Demonstration of bovine viral diarrhea antigen in tissue sections by immunoperoxidase assay. In preparation.

  • Poulsen, F. & T. Hjorth: A simple method for the production of F (ab’)2 preparations by pepsin digestion of total serum protein. Acta path, microbiol. scand. Sect. C 1980, 88, 241–245.

  • Rossi, C. R. & G. K. Kiesel: Bovine immunoglobulin G subclass receptor sites on bovine macrophages. Amer. J. vet. Res. 1977, 38, 1023–1025.

    CAS  PubMed  Google Scholar 

  • Rothlein, R., D. W. Johnson & C. C. Muscoplat: Enumeration and characterization of bovine blood, spleen and lymph node cells containing immunoglobulins. Int. Arch. Allergy 1980, 61, 100–106.

    Article  CAS  Google Scholar 

  • The, T. H. & T. E. W. Feltkamp: Conjugation of fluorescein isothio-cyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 1970, 18, 865–873.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Department of Pathology and Pathological Anatomy, Royal Veterinary and Agricultural University, Copenhagen, Denmark

    Helle Bielefeldt Ohmann

  2. The State Veterinary Institute for Virus Research, Lindholm, Kalvehave, DK-4771, Denmark

    Kristian Dalsgaard

Authors
  1. Helle Bielefeldt Ohmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kristian Dalsgaard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ohmann, H.B., Dalsgaard, K. Indirect Immunofluorescence Using F(ab’)2-Immuno-Reagents for the Demonstration of Bovine Viral Diarrhea Virus (Bvdv) Antigen in Lymphoid Tissue. Acta Vet Scand 21, 705–707 (1980). https://doi.org/10.1186/BF03546858

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03546858

Acta Veterinaria Scandinavica

ISSN: 1751-0147