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A Comparative Investigation of Antibody to Swine Vesicular Disease Virus Using Counter Immunoelectrophoresis, Serum Neutralization and Double Immunodiffusion Tests

En sammenlignende undersøgelse for antistof mod swine vesicular disease virus ved hjælp af counter immunoelektroforese, serum neutralisations- og dobbelt immunodiffusionstest

Abstract

A counter immunoelectrophoresis test (GIET) for assay of antibody to swine vesicular disease virus (SVDV) is described and compared with the serum neutralization test (SNT) and the double immunodiffusion test (DIDT). Using a preparation of complete or empty virus particles as antigen the GIET was found to be more sensitive than the DIDT. The GIET is rapid and economic, and although less sensitive than the SNT it was suggested as an initial large scale screening test for SVD antibody.

Sammendrag

Der er til bestemmelse af antistof mod SVD virus beskrevet en counter immunoelektroforesetest (CIE test), som endvidere er sammenlignet med en serum neutralisationstest (SN test) og en dobbelt immunodiffusionstest (DID test). GIE testen fandtes under anvendelse af en præparation af enten komplette eller tomme virus partikler som antigen mere følsom end DID testen. CIE testen er hurtig og økonomisk, og skønt mindre følsom end SN testen skønnes den egnet som en indledende screening test.

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Sørensen, K.J. A Comparative Investigation of Antibody to Swine Vesicular Disease Virus Using Counter Immunoelectrophoresis, Serum Neutralization and Double Immunodiffusion Tests. Acta Vet Scand 21, 318–323 (1980). https://doi.org/10.1186/BF03546862

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