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Fractionation of Soluble Molybdenum-Binding Proteins from Liver, Kidney, Plasma and Erythrocytes from Sheep Supplemented with Molybdenum
Fraksjonering av løslige molybdenbindende proteiner fra lever, nyre, plasma og erytrocytter fra sau gitt molyb dentilskudd
Acta Veterinaria Scandinavica volume 21, pages 428–437 (1980)
Abstract
Five ewes were given a daily dose of 1.4 mg Mo/kg body weight as ammonium molybdate for 21 to 55 days. Plasma molybdenum concentrations increased considerably following the dosage, but no systematic changes were seen in the plasma copper concentrations. About 80 % of the molybdenum present in blood was found in plasma. With gel filtration it was shown that practically all of the molybdenum present in plasma and red cell haemolyzates was found in a fraction with molecular weight less than 1,500. Soluble proteins from liver and kidney cortex were separated into one high molecular weight fraction with molecular weight above 200,000 and a non-protein fraction with molecular weight less than 1,500. Significant correlations were found between the amounts of molybdenum present in the high molecular weight fraction and the molybdenum concentrations in liver and kidney.
Sammendrag
Fem søyer ble dosert daglig med 1,4 mg Mo/kg kroppsvekt, som ammonium molybdat, i 21 til 55 dager. Det ble funnet en betydelig økning av molybdenmengden i plasma etter dosering, men det ble ikke funnet noen systematisk variasjon i kobbernivået. Omlag 80 % av blodets molybdeninnhold ble funnet i plasma. Ved hjelp av gelfiltrering ble det vist at praktisk talt alt molybdenet i plasma og erytrocytter var bundet i en fraksjon med molekylvekt mindre enn 1.500. I cytosol fra lever og nyre var en del av molybdenet bundet til proteiner med molekylvekt > 200.000, mens resten ble gjenfunnet i en fraksjon med molekylvekt < 1.500. Mengden av proteinbundet molybden økte med innholdet i leveren, mens den relative mengden av proteinbundet molybden avtok med stigende molybdennivà både i lever og nyre.
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This work was supported by grants from the Agricultural Research Council of Norway.
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Norheim, G., Søli, N.E., Frøslie, A. et al. Fractionation of Soluble Molybdenum-Binding Proteins from Liver, Kidney, Plasma and Erythrocytes from Sheep Supplemented with Molybdenum. Acta Vet Scand 21, 428–437 (1980). https://doi.org/10.1186/BF03546875
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DOI: https://doi.org/10.1186/BF03546875